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Literature summary extracted from

  • London, J.; Hausman, S.Z.; Thompson, J.
    Characterization of a membrane-regulated sugar phosphate phosphohydrolase from Lactobacillus casei (1985), J. Bacteriol., 163, 951-956.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.58 Fe2+
-
 Lacticaseibacillus casei
3.1.3.58 Zn2+
-
 Lacticaseibacillus casei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.58 cytoplasm phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane Lacticaseibacillus casei 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.58 Co2+ 5 mM, divalent cation required for maximal activity, doubles the rate of glucose 6-phosphate hydrolysis Lacticaseibacillus casei
3.1.3.58 Mg2+ 5 mM, divalent cation required for maximal activity, triples the rate of glucose 6-phosphate hydrolysis Lacticaseibacillus casei
3.1.3.58 additional information Mn2+ and Ni2+ have no effect on enzyme activity Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.58 32000
-
 2 * 32000, SDS-PAGE Lacticaseibacillus casei
3.1.3.58 62000
-
 gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.58 D-glucose 6-phosphate + H2O Lacticaseibacillus casei key component of the futile xylitol cycle D-glucose + phosphate
-
 ?
3.1.3.58 D-xylitol-5-phosphate + H2O Lacticaseibacillus casei relative activity 34% D-xylitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.58 Lacticaseibacillus casei
-
 Cl-16
-
3.1.3.58 Lacticaseibacillus casei Cl-16
-
 Cl-16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.58 partial Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.58 0.154
-
 2-deoxyglucose 6-phosphate as substrate Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.58 2-Deoxy-D-glucose 6-phosphate + H2O relative activity 75% Lacticaseibacillus casei 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.58 2-Deoxy-D-glucose 6-phosphate + H2O relative activity 75% Lacticaseibacillus casei Cl-16 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.58 3-phosphoglycerate + H2O relative activity 4% Lacticaseibacillus casei glycerate + phosphate
-
 ?
3.1.3.58 3-phosphoglycerate + H2O relative activity 4% Lacticaseibacillus casei Cl-16 glycerate + phosphate
-
 ?
3.1.3.58 6-phosphogluconate + H2O relative activity 69% Lacticaseibacillus casei gluconate + phosphate
-
 ?
3.1.3.58 alpha-glycerophosphate + H2O relative activity 50% Lacticaseibacillus casei glycerol + phosphate
-
 ?
3.1.3.58 beta-D-glucose-1,6-diphosphate + H2O relative activity 56% Lacticaseibacillus casei beta-D-glucose + phosphate
-
 ?
3.1.3.58 D-erythrose-4-phosphate + H2O relative activity 69% Lacticaseibacillus casei D-erythrose + phosphate
-
 ?
3.1.3.58 D-fructose-1,6-diphosphate + H2O relative activity 16% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
3.1.3.58 D-fructose-1-phosphate + H2O relative activity 8% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
3.1.3.58 D-fructose-6-phosphate + H2O relative activity 47% Lacticaseibacillus casei D-fructose + phosphate
-
 ?
3.1.3.58 D-galactose-1-phosphate + H2O relative activity 8% Lacticaseibacillus casei D-galactose + phosphate
-
 ?
3.1.3.58 D-galactose-6-phosphate + H2O relative activity 11% Lacticaseibacillus casei D-galactose + phosphate
-
 ?
3.1.3.58 D-glucosamine-6-phosphate + H2O relative activity 28% Lacticaseibacillus casei D-glucosamine + phosphate
-
 ?
3.1.3.58 D-glucose 6-phosphate + H2O relative activity 100% Lacticaseibacillus casei D-glucose + phosphate
-
 ?
3.1.3.58 D-glucose 6-phosphate + H2O key component of the futile xylitol cycle Lacticaseibacillus casei D-glucose + phosphate
-
 ?
3.1.3.58 D-glucose 6-phosphate + H2O relative activity 100% Lacticaseibacillus casei Cl-16 D-glucose + phosphate
-
 ?
3.1.3.58 D-glucose-1-phosphate + H2O relative activity 3% Lacticaseibacillus casei D-glucose + phosphate
-
 ?
3.1.3.58 D-mannose 6-phosphate + H2O relative activity 80% Lacticaseibacillus casei D-mannose + phosphate
-
 ?
3.1.3.58 D-ribitol-5-phosphate + H2O relative activity 15% Lacticaseibacillus casei D-ribitol + phosphate
-
 ?
3.1.3.58 D-ribose 5-phosphate + H2O relative activity 53% Lacticaseibacillus casei D-ribose + phosphate
-
 ?
3.1.3.58 D-ribose 5-phosphate + H2O relative activity 53% Lacticaseibacillus casei Cl-16 D-ribose + phosphate
-
 ?
3.1.3.58 D-sedoheptulose-7-phosphate + H2O relative activity 34% Lacticaseibacillus casei sedoheptulose + phosphate
-
 ?
3.1.3.58 D-sorbitol-6-phosphate + H2O relative activity 33% Lacticaseibacillus casei D-sorbitol + phosphate
-
 ?
3.1.3.58 D-xylitol-5-phosphate + H2O relative activity 34% Lacticaseibacillus casei D-xylitol + phosphate
-
 ?
3.1.3.58 D-xylose-1-phosphate + H2O relative activity 3% Lacticaseibacillus casei D-xylose + phosphate
-
 ?
3.1.3.58 mannitol-1-phosphate + H2O relative activity 16% Lacticaseibacillus casei D-mannitol + phosphate
-
 ?
3.1.3.58 additional information little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate Lacticaseibacillus casei ?
-
 ?
3.1.3.58 additional information little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate Lacticaseibacillus casei Cl-16 ?
-
 ?
3.1.3.58 o-nitrophenyl D-galactose-6-phosphate + H2O relative activity 6% Lacticaseibacillus casei o-nitrophenyl D-galactose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.58 dimer 2 * 32000, SDS-PAGE Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.58 5.5 6
-
 Lacticaseibacillus casei