BRENDA - Enzyme Database

Biochemical evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin

Kim, B.S.; Cropp, T.A.; Beck, B.J.; Sherman, D.H.; Reynolds, K.A.; J. Biol. Chem. 277, 48028-48034 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.2
expression in Escherichia coli BL21 as His-tagged protein
Streptomyces venezuelae
3.1.2.20
expression in Escherichia coli BL21 as His-tagged protein
Streptomyces venezuelae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
additional information
Streptomyces venezuelae
involved in the biosynthesis of the polyketide pikromycin
?
-
-
-
3.1.2.20
additional information
Streptomyces venezuelae
involved in the biosynthesis of the polyketide pikromycin
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.2
Streptomyces venezuelae
Q9ZGI1
thioesterase II, gene pikAV
-
3.1.2.20
Streptomyces venezuelae
Q9ZGI1
thioesterase II, gene pikAV
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.2
recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity
Streptomyces venezuelae
3.1.2.20
recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity
Streptomyces venezuelae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.2
acetyl-CoA + H2O
poor substrate
646202
Streptomyces venezuelae
CoA + acetate
-
646202
Streptomyces venezuelae
?
3.1.2.2
acyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + a carboxylate
-
-
-
?
3.1.2.2
butyryl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
butanoate + acyl-carrier protein
-
-
-
?
3.1.2.2
malonyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + malonate
-
646202
Streptomyces venezuelae
?
3.1.2.2
additional information
involved in the biosynthesis of the polyketide pikromycin
646202
Streptomyces venezuelae
?
-
-
-
-
3.1.2.2
n-butyryl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + n-butanoate
-
-
-
?
3.1.2.2
propionyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + propionate
-
646202
Streptomyces venezuelae
?
3.1.2.2
propionyl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
propionate + acyl-carrier protein
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
poor substrate
646202
Streptomyces venezuelae
CoA + acetate
-
646202
Streptomyces venezuelae
?
3.1.2.20
acyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + a carboxylate
-
-
-
?
3.1.2.20
butyryl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
butanoate + acyl-carrier protein
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + malonate
-
646202
Streptomyces venezuelae
?
3.1.2.20
additional information
involved in the biosynthesis of the polyketide pikromycin
646202
Streptomyces venezuelae
?
-
-
-
-
3.1.2.20
n-butyryl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + n-butanoate
-
-
-
?
3.1.2.20
propionyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + propionate
-
646202
Streptomyces venezuelae
?
3.1.2.20
propionyl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
propionate + acyl-carrier protein
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.2
expression in Escherichia coli BL21 as His-tagged protein
Streptomyces venezuelae
3.1.2.20
expression in Escherichia coli BL21 as His-tagged protein
Streptomyces venezuelae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
additional information
Streptomyces venezuelae
involved in the biosynthesis of the polyketide pikromycin
?
-
-
-
3.1.2.20
additional information
Streptomyces venezuelae
involved in the biosynthesis of the polyketide pikromycin
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.2
recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity
Streptomyces venezuelae
3.1.2.20
recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity
Streptomyces venezuelae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.2
acetyl-CoA + H2O
poor substrate
646202
Streptomyces venezuelae
CoA + acetate
-
646202
Streptomyces venezuelae
?
3.1.2.2
acyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + a carboxylate
-
-
-
?
3.1.2.2
butyryl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
butanoate + acyl-carrier protein
-
-
-
?
3.1.2.2
malonyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + malonate
-
646202
Streptomyces venezuelae
?
3.1.2.2
additional information
involved in the biosynthesis of the polyketide pikromycin
646202
Streptomyces venezuelae
?
-
-
-
-
3.1.2.2
n-butyryl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + n-butanoate
-
-
-
?
3.1.2.2
propionyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + propionate
-
646202
Streptomyces venezuelae
?
3.1.2.2
propionyl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
propionate + acyl-carrier protein
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
poor substrate
646202
Streptomyces venezuelae
CoA + acetate
-
646202
Streptomyces venezuelae
?
3.1.2.20
acyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + a carboxylate
-
-
-
?
3.1.2.20
butyryl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
butanoate + acyl-carrier protein
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + malonate
-
646202
Streptomyces venezuelae
?
3.1.2.20
additional information
involved in the biosynthesis of the polyketide pikromycin
646202
Streptomyces venezuelae
?
-
-
-
-
3.1.2.20
n-butyryl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + n-butanoate
-
-
-
?
3.1.2.20
propionyl-CoA + H2O
-
646202
Streptomyces venezuelae
CoA + propionate
-
646202
Streptomyces venezuelae
?
3.1.2.20
propionyl-[acyl-carrier protein] + H2O
-
646202
Streptomyces venezuelae
propionate + acyl-carrier protein
-
-
-
?