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Literature summary extracted from

  • Hunt, M.C.; Solaas, K.; Kase, B.F.; Alexson, S.E.
    Characterization of an acyl-coA thioesterase that functions as a major regulator of peroxisomal lipid metabolism (2002), J. Biol. Chem., 277, 1128-1138.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 additional information fasting induces enzyme expression, dependent on peroxisome proliferator-activated receptor alpha Mus musculus
3.1.2.2 peroxisome proliferator WY-14,643 induction of enzyme expression, dependent on peroxisome proliferator-activated receptor alpha Mus musculus
3.1.2.2 PPARalpha internal receptor protein essential for effect of stimulating compounds Mus musculus
3.1.2.2 PPARalpha i.e. peroxisome proliferator-activated receptor alpha Mus musculus
3.1.2.20 PPARalpha internal receptor protein essential for effect of stimulating compounds Mus musculus
3.1.2.20 PPARalpha i.e. peroxisome proliferator-activated receptor alpha Mus musculus
3.1.2.27 additional information fasting induces the enzyme Mus musculus
3.1.2.27 PPARII peroxisomal proliferator activated receptor-II activates the enzyme, regulatory function, fasting-mediated induction in vivo, dependent on the peroxisomal proliferator WY-14,643 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) Mus musculus
3.1.2.2 isozyme PTE-2, DNA sequence determination and analysis Homo sapiens
3.1.2.2 isozyme PTE-2, DNA sequence determination and analysis, expression as green-fluorescent-protein fusion protein in human skin fibroblasts Mus musculus
3.1.2.20 DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) Mus musculus
3.1.2.27 gene PTE-2, DNA and amino acid sequence determination and analysis, location on chromosome 20q12-q13, expression in Escherichia coli, expression as GFP-fusion protein in human skin fibroblasts showing targeting to the peroxisomes Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 acyl-CoA substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects Mus musculus
3.1.2.2 CoA intraperoxisomal regulatory role Mus musculus
3.1.2.20 acyl-CoA substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects Mus musculus
3.1.2.27 4-chloromercuribenzoate 50% inhibition at 0.001 mM Mus musculus
3.1.2.27 acyl-CoA enzyme is substrate-inhibited at 0.005-0.01 mM of acyl-CoAs with chain length longer than C10 Mus musculus
3.1.2.27 CoA product inhibition with regulatory function, 50% inhibition at 0.01-0.015 mM Mus musculus
3.1.2.27 DTNB 50% inhibition at 0.15 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 additional information
-
 additional information
-
 Mus musculus
3.1.2.2 additional information
-
 additional information diverse substrates, kinetics Mus musculus
3.1.2.2 0.0014
-
 palmitoleoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0017
-
 hexadecanoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0023
-
 linoleoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0025
-
 tetradecanoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0042
-
 arachidoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0063
-
 trihydroxycoprostanoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0067
-
 Arachidonoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0088
-
 chenodeoxycholoyl-CoA pH 7.4 Mus musculus
3.1.2.2 0.0146
-
 choloyl-CoA pH 7.4 Mus musculus
3.1.2.20 additional information
-
 additional information
-
 Mus musculus
3.1.2.20 0.0014
-
 palmitoleoyl-CoA pH 7.4 Mus musculus
3.1.2.20 0.0017
-
 hexadecanoyl-CoA pH 7.4 Mus musculus
3.1.2.20 0.0023
-
 linoleoyl-CoA pH 7.4 Mus musculus
3.1.2.20 0.0025
-
 tetradecanoyl-CoA pH 7.4 Mus musculus
3.1.2.20 0.0042
-
 arachidoyl-CoA pH 7.4 Mus musculus
3.1.2.20 0.0067
-
 Arachidonoyl-CoA pH 7.4 Mus musculus
3.1.2.27 additional information
-
 additional information
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 peroxisome isozyme PTE-II Mus musculus 5777
-
3.1.2.2 peroxisome isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes Homo sapiens 5777
-
3.1.2.2 peroxisome isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes Mus musculus 5777
-
3.1.2.20 peroxisome isozyme PTE-II Mus musculus 5777
-
3.1.2.27 peroxisome
-
 Mus musculus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.27 35886
-
 2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE Mus musculus
3.1.2.27 36000
-
 2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE Mus musculus
3.1.2.27 70000
-
 recombinant enzyme, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 4,8-dimethylnonanoyl-CoA + H2O Mus musculus
-
 CoA + 4,8-dimethylnonanoate
-
 ?
3.1.2.2 additional information Homo sapiens enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism ?
-
 ?
3.1.2.2 additional information Mus musculus enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism ?
-
 ?
3.1.2.20 4,8-dimethylnonanoyl-CoA + H2O Mus musculus
-
 CoA + 4,8-dimethylnonanoate
-
 ?
3.1.2.27 2-trans-decenoyl-CoA + H2O Mus musculus substrate is a beta-oxidation intermediate, low activity 2-trans-decenoate + CoA
-
 ?
3.1.2.27 3-hydroxypalmitoyl-CoA + H2O Mus musculus substrate is a beta-oxidation intermediate, low activity 3-hydroxypalmitate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O Mus musculus
-
 chenodeoxycholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O Mus musculus
-
 cholate + CoA
-
 ?
3.1.2.27 hydroxymethylglutaryl-CoA + H2O Mus musculus
-
 hydroxymethylglutarate + CoA
-
 ?
3.1.2.27 additional information Mus musculus the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates ?
-
 ?
3.1.2.27 trihydroxycoprostanoyl-CoA + H2O Mus musculus
-
 trihydroxycoprostanate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Homo sapiens O14734
-
-
3.1.2.2 Mus musculus P58137 gene pte-2, isozyme PTE-II
-
3.1.2.2 Mus musculus P58137 adult male C57 BL/6 mice, peroxisomal isozyme PTE-2
-
3.1.2.20 Mus musculus P58137 gene pte-2, isozyme PTE-II
-
3.1.2.27 Mus musculus P58137
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 recombinant from Escherichia coli Mus musculus
3.1.2.20 recombinant from Escherichia coli Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.2 palmitoyl-CoA + H2O = CoA + palmitate catalytic triad D233-S255-Q305 Mus musculus
a
3.1.2.20 an acyl-CoA + H2O = CoA + a carboxylate catalytic triad D233-S255-Q305 Mus musculus
a
3.1.2.27 choloyl-CoA + H2O = cholate + CoA the catalytic triad is formed by Asp233, Ser255, and Gln305 Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 heart
-
 Mus musculus
-
3.1.2.2 kidney
-
 Mus musculus
-
3.1.2.2 liver
-
 Mus musculus
-
3.1.2.2 additional information ubiquitous expression Mus musculus
-
3.1.2.2 muscle
-
 Mus musculus
-
3.1.2.2 testis
-
 Mus musculus
-
3.1.2.20 additional information ubiquitous expression Mus musculus
-
3.1.2.27 heart lower enzyme content Mus musculus
-
3.1.2.27 kidney high enzyme content Mus musculus
-
3.1.2.27 liver
-
 Mus musculus
-
3.1.2.27 muscle lower enzyme content Mus musculus
-
3.1.2.27 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 2-methylstearoyl-CoA + H2O
-
 Mus musculus CoA + 2-methylstearate
-
 ?
3.1.2.2 2-trans-decenoyl-CoA + H2O
-
 Mus musculus CoA + 2-trans-decenoate
-
 ?
3.1.2.2 3-hydroxy-3-methyl glutaryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxy-3-methyl glutarate
-
 ?
3.1.2.2 3-hydroxy-3-methylglutaryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxy-3-methylglutarate
-
 ?
3.1.2.2 3-hydroxyhexadecanoyl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxyhexadecanoate
-
 ?
3.1.2.2 3-hydroxypalmitoyl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxypalmitate
-
 ?
3.1.2.2 4,8-dimethylnonanoyl-CoA + H2O
-
 Mus musculus CoA + 4,8-dimethylnonanoate
-
 ?
3.1.2.2 acetoacetyl-CoA + H2O
-
 Mus musculus CoA + acetoacetate
-
 ?
3.1.2.2 acetyl-CoA + H2O
-
 Mus musculus CoA + acetate
-
 ?
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Mus musculus CoA + a carboxylate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O
-
 Mus musculus CoA + arachidonate
-
 ?
3.1.2.2 arachidoyl-CoA + H2O
-
 Mus musculus CoA + arachidate
-
 ?
3.1.2.2 butyryl-CoA + H2O
-
 Mus musculus butanoate + CoA
-
 ?
3.1.2.2 chenodeoxycholoyl-CoA + H2O activity is PPARalpha-inducible Mus musculus CoA + chenodeoxycholoate
-
 ?
3.1.2.2 chenodeoxycholoyl-CoA + H2O bile acid derivative substrate Mus musculus CoA + chenodeoxycholoate
-
 ?
3.1.2.2 chenodeoxycholoyl-CoA + H2O
-
 Mus musculus CoA + chenodeoxycholate
-
 ?
3.1.2.2 choloyl-CoA + H2O activity is PPARalpha-inducible Mus musculus CoA + choloate
-
 ?
3.1.2.2 choloyl-CoA + H2O bile acid derivative substrate Mus musculus CoA + choloate
-
 ?
3.1.2.2 choloyl-CoA + H2O best substrate Mus musculus CoA + cholate
-
 ?
3.1.2.2 clofibroyl-CoA + H2O
-
 Mus musculus CoA + clofibrate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Mus musculus CoA + decanoate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O
-
 Mus musculus CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O
-
 Mus musculus CoA + hexadecanoate
-
 ?
3.1.2.2 hexanoyl-CoA + H2O
-
 Mus musculus CoA + hexanoate
-
 ?
3.1.2.2 hexanoyl-CoA + H2O
-
 Mus musculus CoA + n-hexanoate
-
 ?
3.1.2.2 lauroyl-CoA + H2O
-
 Mus musculus CoA + laurate
-
 ?
3.1.2.2 linoleoyl-CoA + H2O
-
 Mus musculus CoA + linoleate
-
 ?
3.1.2.2 malonyl-CoA + H2O
-
 Mus musculus CoA + malonate
-
 ?
3.1.2.2 additional information broad chain length specificity Mus musculus ?
-
 ?
3.1.2.2 additional information enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism Homo sapiens ?
-
 ?
3.1.2.2 additional information enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism Mus musculus ?
-
 ?
3.1.2.2 myristoleoyl-CoA + H2O
-
 Mus musculus myristoleoate + CoA
-
 ?
3.1.2.2 myristoyl-CoA + H2O
-
 Mus musculus CoA + myristate
-
 ?
3.1.2.2 n-butyryl-CoA + H2O
-
 Mus musculus CoA + n-butanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O
-
 Mus musculus CoA + octadecanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Mus musculus CoA + octanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Mus musculus CoA + oleate
-
 ?
3.1.2.2 palmitoleoyl-CoA + H2O
-
 Mus musculus CoA + palmitoleoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Homo sapiens CoA + palmitate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Mus musculus CoA + palmitate
-
 ?
3.1.2.2 propionyl-CoA + H2O
-
 Mus musculus CoA + propionate
-
 ?
3.1.2.2 prostaglandin F2alpha + H2O
-
 Mus musculus ?
-
 ?
3.1.2.2 prostaglandin F2alpha-CoA + H2O
-
 Mus musculus prostaglandin F2alpha + CoA
-
 ?
3.1.2.2 stearoyl-CoA + H2O
-
 Mus musculus CoA + stearate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O
-
 Mus musculus CoA + tetradecanoate
-
 ?
3.1.2.2 trihydroxycoprostanoyl-CoA + H2O
-
 Mus musculus CoA + trihydroxycoprostanoate
-
 ?
3.1.2.20 2-methylstearoyl-CoA + H2O
-
 Mus musculus CoA + 2-methylstearate
-
 ?
3.1.2.20 2-trans-decenoyl-CoA + H2O
-
 Mus musculus CoA + 2-trans-decenoate
-
 ?
3.1.2.20 3-hydroxy-3-methyl glutaryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxy-3-methyl glutarate
-
 ?
3.1.2.20 3-hydroxyhexadecanoyl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxyhexadecanoate
-
 ?
3.1.2.20 4,8-dimethylnonanoyl-CoA + H2O
-
 Mus musculus CoA + 4,8-dimethylnonanoate
-
 ?
3.1.2.20 acetoacetyl-CoA + H2O
-
 Mus musculus CoA + acetoacetate
-
 ?
3.1.2.20 acetyl-CoA + H2O
-
 Mus musculus CoA + acetate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Mus musculus CoA + a carboxylate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Mus musculus CoA + arachidonate
-
 ?
3.1.2.20 arachidoyl-CoA + H2O
-
 Mus musculus CoA + arachidate
-
 ?
3.1.2.20 chenodeoxycholoyl-CoA + H2O activity is PPARalpha-inducible Mus musculus CoA + chenodeoxycholoate
-
 ?
3.1.2.20 chenodeoxycholoyl-CoA + H2O bile acid derivative substrate Mus musculus CoA + chenodeoxycholoate
-
 ?
3.1.2.20 choloyl-CoA + H2O activity is PPARalpha-inducible Mus musculus CoA + choloate
-
 ?
3.1.2.20 choloyl-CoA + H2O bile acid derivative substrate Mus musculus CoA + choloate
-
 ?
3.1.2.20 clofibroyl-CoA + H2O
-
 Mus musculus CoA + clofibrate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Mus musculus CoA + decanoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O
-
 Mus musculus CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O
-
 Mus musculus CoA + hexadecanoate
-
 ?
3.1.2.20 hexanoyl-CoA + H2O
-
 Mus musculus CoA + hexanoate
-
 ?
3.1.2.20 linoleoyl-CoA + H2O
-
 Mus musculus CoA + linoleate
-
 ?
3.1.2.20 malonyl-CoA + H2O
-
 Mus musculus CoA + malonate
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Mus musculus CoA + myristate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Mus musculus CoA + n-butanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O
-
 Mus musculus CoA + octadecanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Mus musculus CoA + octanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Mus musculus CoA + oleate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Mus musculus CoA + palmitoleoate
-
 ?
3.1.2.20 propionyl-CoA + H2O
-
 Mus musculus CoA + propionate
-
 ?
3.1.2.20 prostaglandin F2alpha-CoA + H2O
-
 Mus musculus prostaglandin F2alpha + CoA
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O
-
 Mus musculus CoA + tetradecanoate
-
 ?
3.1.2.20 trihydroxycoprostanoyl-CoA + H2O
-
 Mus musculus CoA + trihydroxycoprostanoate
-
 ?
3.1.2.27 2-trans-decenoyl-CoA + H2O substrate is a beta-oxidation intermediate, low activity Mus musculus 2-trans-decenoate + CoA
-
 ?
3.1.2.27 3-hydroxypalmitoyl-CoA + H2O substrate is a beta-oxidation intermediate, low activity Mus musculus 3-hydroxypalmitate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O
-
 Mus musculus chenodeoxycholate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O best substrate Mus musculus chenodeoxycholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O
-
 Mus musculus cholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O best substrate Mus musculus cholate + CoA
-
 ?
3.1.2.27 hydroxymethylglutaryl-CoA + H2O
-
 Mus musculus hydroxymethylglutarate + CoA
-
 ?
3.1.2.27 additional information the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates Mus musculus ?
-
 ?
3.1.2.27 additional information the enzyme shows broad specificity for substrates and (branched) acyl-CoA chain lengths Mus musculus ?
-
 ?
3.1.2.27 trihydroxycoprostanoyl-CoA + H2O
-
 Mus musculus trihydroxycoprostanate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.27 dimer 2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 peroxisomal acyl-CoA thioesterase 2
-
 Homo sapiens
3.1.2.2 peroxisomal acyl-CoA thioesterase 2
-
 Mus musculus
3.1.2.2 PTE-2
-
 Homo sapiens
3.1.2.2 PTE-2
-
 Mus musculus
3.1.2.27 acyl-CoA thioesterase
-
 Mus musculus
3.1.2.27 peroxisomal acyl-coA thioesterase
-
 Mus musculus
3.1.2.27 PTE-2
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.4
-
 assay at Mus musculus
3.1.2.20 7.4
-
 assay at Mus musculus
3.1.2.27 7.4
-
 assay at Mus musculus