BRENDA - Enzyme Database

Characterization of an acyl-coA thioesterase that functions as a major regulator of peroxisomal lipid metabolism

Hunt, M.C.; Solaas, K.; Kase, B.F.; Alexson, S.E.; J. Biol. Chem. 277, 1128-1138 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.2
additional information
fasting induces enzyme expression, dependent on peroxisome proliferator-activated receptor alpha
Mus musculus
3.1.2.2
peroxisome proliferator WY-14,643
induction of enzyme expression, dependent on peroxisome proliferator-activated receptor alpha
Mus musculus
3.1.2.2
PPARalpha
i.e. peroxisome proliferator-activated receptor alpha; internal receptor protein essential for effect of stimulating compounds
Mus musculus
3.1.2.20
PPARalpha
i.e. peroxisome proliferator-activated receptor alpha; internal receptor protein essential for effect of stimulating compounds
Mus musculus
3.1.2.27
additional information
fasting induces the enzyme
Mus musculus
3.1.2.27
PPARII
peroxisomal proliferator activated receptor-II activates the enzyme, regulatory function, fasting-mediated induction in vivo, dependent on the peroxisomal proliferator WY-14,643
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.2
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3); isozyme PTE-2, DNA sequence determination and analysis, expression as green-fluorescent-protein fusion protein in human skin fibroblasts
Mus musculus
3.1.2.2
isozyme PTE-2, DNA sequence determination and analysis
Homo sapiens
3.1.2.20
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3)
Mus musculus
3.1.2.27
gene PTE-2, DNA and amino acid sequence determination and analysis, location on chromosome 20q12-q13, expression in Escherichia coli, expression as GFP-fusion protein in human skin fibroblasts showing targeting to the peroxisomes
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.2
acyl-CoA
substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects
Mus musculus
3.1.2.2
CoA
intraperoxisomal regulatory role
Mus musculus
3.1.2.20
acyl-CoA
substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects
Mus musculus
3.1.2.27
4-chloromercuribenzoate
50% inhibition at 0.001 mM
Mus musculus
3.1.2.27
acyl-CoA
enzyme is substrate-inhibited at 0.005-0.01 mM of acyl-CoAs with chain length longer than C10
Mus musculus
3.1.2.27
CoA
product inhibition with regulatory function, 50% inhibition at 0.01-0.015 mM
Mus musculus
3.1.2.27
DTNB
50% inhibition at 0.15 mM
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.2
additional information
-
additional information
; diverse substrates, kinetics
Mus musculus
3.1.2.2
0.0014
-
palmitoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0017
-
hexadecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0023
-
linoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0025
-
tetradecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0042
-
arachidoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0063
-
trihydroxycoprostanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0067
-
Arachidonoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0088
-
chenodeoxycholoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0146
-
choloyl-CoA
pH 7.4
Mus musculus
3.1.2.20
additional information
-
additional information
-
Mus musculus
3.1.2.20
0.0014
-
palmitoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0017
-
hexadecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0023
-
linoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0025
-
tetradecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0042
-
arachidoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0067
-
Arachidonoyl-CoA
pH 7.4
Mus musculus
3.1.2.27
additional information
-
additional information
-
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.2
peroxisome
isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes
Homo sapiens
5777
-
3.1.2.2
peroxisome
isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes; isozyme PTE-II
Mus musculus
5777
-
3.1.2.20
peroxisome
isozyme PTE-II
Mus musculus
5777
-
3.1.2.27
peroxisome
-
Mus musculus
5777
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.27
35886
-
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
3.1.2.27
36000
-
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
3.1.2.27
70000
-
recombinant enzyme, gel filtration
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
Mus musculus
-
CoA + 4,8-dimethylnonanoate
-
-
?
3.1.2.2
additional information
Homo sapiens
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
?
-
Homo sapiens
?
3.1.2.2
additional information
Mus musculus
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
?
-
Mus musculus
?
3.1.2.20
4,8-dimethylnonanoyl-CoA + H2O
Mus musculus
-
CoA + 4,8-dimethylnonanoate
-
-
?
3.1.2.27
2-trans-decenoyl-CoA + H2O
Mus musculus
substrate is a beta-oxidation intermediate, low activity
2-trans-decenoate + CoA
-
-
?
3.1.2.27
3-hydroxypalmitoyl-CoA + H2O
Mus musculus
substrate is a beta-oxidation intermediate, low activity
3-hydroxypalmitate + CoA
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
Mus musculus
-
chenodeoxycholate + CoA
-
-
?
3.1.2.27
choloyl-CoA + H2O
Mus musculus
-
cholate + CoA
-
-
?
3.1.2.27
hydroxymethylglutaryl-CoA + H2O
Mus musculus
-
hydroxymethylglutarate + CoA
-
-
?
3.1.2.27
additional information
Mus musculus
the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates
?
-
-
-
3.1.2.27
trihydroxycoprostanoyl-CoA + H2O
Mus musculus
-
trihydroxycoprostanate + CoA
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.2
Homo sapiens
O14734
-
-
3.1.2.2
Mus musculus
P58137
adult male C57 BL/6 mice, peroxisomal isozyme PTE-2; gene pte-2, isozyme PTE-II
-
3.1.2.20
Mus musculus
P58137
gene pte-2, isozyme PTE-II
-
3.1.2.27
Mus musculus
P58137
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.2
recombinant from Escherichia coli
Mus musculus
3.1.2.20
recombinant from Escherichia coli
Mus musculus
Reaction
EC Number
Reaction
Commentary
Organism
3.1.2.2
palmitoyl-CoA + H2O = CoA + palmitate
catalytic triad D233-S255-Q305
Mus musculus
3.1.2.20
acyl-CoA + H2O = CoA + a carboxylate
catalytic triad D233-S255-Q305
Mus musculus
3.1.2.27
choloyl-CoA + H2O = cholate + CoA
the catalytic triad is formed by Asp233, Ser255, and Gln305
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.2
heart
-
Mus musculus
-
3.1.2.2
kidney
-
Mus musculus
-
3.1.2.2
liver
-
Mus musculus
-
3.1.2.2
additional information
ubiquitous expression
Mus musculus
-
3.1.2.2
muscle
-
Mus musculus
-
3.1.2.2
testis
-
Mus musculus
-
3.1.2.20
additional information
ubiquitous expression
Mus musculus
-
3.1.2.27
heart
lower enzyme content
Mus musculus
-
3.1.2.27
kidney
high enzyme content
Mus musculus
-
3.1.2.27
liver
-
Mus musculus
-
3.1.2.27
muscle
lower enzyme content
Mus musculus
-
3.1.2.27
testis
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.2
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
-
-
?
3.1.2.2
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
646200
Mus musculus
?
3.1.2.2
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
-
-
?
3.1.2.2
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
646200
Mus musculus
?
3.1.2.2
3-hydroxy-3-methyl glutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methyl glutarate
-
-
-
?
3.1.2.2
3-hydroxy-3-methylglutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methylglutarate
-
646200
Mus musculus
?
3.1.2.2
3-hydroxyhexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxyhexadecanoate
-
-
-
?
3.1.2.2
3-hydroxypalmitoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxypalmitate
-
646200
Mus musculus
?
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
-
-
?
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
646200
Mus musculus
?
3.1.2.2
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.2
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
646200
Mus musculus
?
3.1.2.2
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.2
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
646200
Mus musculus
?
3.1.2.2
acyl-CoA + H2O
long-chain fatty-acyl-CoA
646200
Mus musculus
CoA + a carboxylate
-
-
-
?
3.1.2.2
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
-
-
?
3.1.2.2
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
646200
Mus musculus
?
3.1.2.2
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
-
-
?
3.1.2.2
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
646200
Mus musculus
?
3.1.2.2
butyryl-CoA + H2O
-
646200
Mus musculus
butanoate + CoA
-
646200
Mus musculus
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
-
646200
Mus musculus
CoA + chenodeoxycholate
-
646200
Mus musculus
?
3.1.2.2
choloyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.2
choloyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.2
choloyl-CoA + H2O
best substrate
646200
Mus musculus
CoA + cholate
-
646200
Mus musculus
?
3.1.2.2
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
-
-
?
3.1.2.2
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
646200
Mus musculus
?
3.1.2.2
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
-
-
?
3.1.2.2
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
646200
Mus musculus
?
3.1.2.2
dodecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + dodecanoate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexanoate
-
-
-
?
3.1.2.2
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + n-hexanoate
-
646200
Mus musculus
?
3.1.2.2
lauroyl-CoA + H2O
-
646200
Mus musculus
CoA + laurate
-
646200
Mus musculus
?
3.1.2.2
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
-
-
?
3.1.2.2
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
646200
Mus musculus
?
3.1.2.2
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
-
-
?
3.1.2.2
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
646200
Mus musculus
?
3.1.2.2
additional information
broad chain length specificity
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
additional information
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
646200
Homo sapiens
?
-
646200
Homo sapiens
?
3.1.2.2
additional information
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
myristoleoyl-CoA + H2O
-
646200
Mus musculus
myristoleoate + CoA
-
646200
Mus musculus
?
3.1.2.2
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
-
-
?
3.1.2.2
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
646200
Mus musculus
?
3.1.2.2
n-butyryl-CoA + H2O
-
646200
Mus musculus
CoA + n-butanoate
-
-
-
?
3.1.2.2
octadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octadecanoate
-
-
-
?
3.1.2.2
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
-
-
?
3.1.2.2
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
646200
Mus musculus
?
3.1.2.2
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
-
-
?
3.1.2.2
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
646200
Mus musculus
?
3.1.2.2
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
-
-
?
3.1.2.2
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
646200
Mus musculus
?
3.1.2.2
palmitoyl-CoA + H2O
-
646200
Homo sapiens
CoA + palmitate
-
646200
Homo sapiens
?
3.1.2.2
palmitoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitate
-
646200
Mus musculus
?
3.1.2.2
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
-
-
?
3.1.2.2
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
646200
Mus musculus
?
3.1.2.2
prostaglandin F2alpha + H2O
-
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
prostaglandin F2alpha-CoA + H2O
-
646200
Mus musculus
prostaglandin F2alpha + CoA
-
-
-
?
3.1.2.2
stearoyl-CoA + H2O
-
646200
Mus musculus
CoA + stearate
-
646200
Mus musculus
?
3.1.2.2
tetradecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + tetradecanoate
-
-
-
?
3.1.2.2
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
-
-
?
3.1.2.2
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
646200
Mus musculus
?
3.1.2.20
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
-
-
?
3.1.2.20
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
-
-
?
3.1.2.20
3-hydroxy-3-methyl glutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methyl glutarate
-
-
-
?
3.1.2.20
3-hydroxyhexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxyhexadecanoate
-
-
-
?
3.1.2.20
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
-
-
?
3.1.2.20
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.20
acyl-CoA + H2O
long-chain fatty-acyl-CoA
646200
Mus musculus
CoA + a carboxylate
-
-
-
?
3.1.2.20
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
-
-
?
3.1.2.20
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
-
-
?
3.1.2.20
chenodeoxycholoyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.20
chenodeoxycholoyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.20
choloyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.20
choloyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.20
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
-
-
?
3.1.2.20
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
-
-
?
3.1.2.20
dodecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + dodecanoate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexanoate
-
-
-
?
3.1.2.20
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
-
-
?
3.1.2.20
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
-
-
?
3.1.2.20
n-butyryl-CoA + H2O
-
646200
Mus musculus
CoA + n-butanoate
-
-
-
?
3.1.2.20
octadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octadecanoate
-
-
-
?
3.1.2.20
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
-
-
?
3.1.2.20
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
-
-
?
3.1.2.20
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
-
-
?
3.1.2.20
prostaglandin F2alpha-CoA + H2O
-
646200
Mus musculus
prostaglandin F2alpha + CoA
-
-
-
?
3.1.2.20
tetradecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + tetradecanoate
-
-
-
?
3.1.2.20
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
-
-
?
3.1.2.27
2-trans-decenoyl-CoA + H2O
substrate is a beta-oxidation intermediate, low activity
646200
Mus musculus
2-trans-decenoate + CoA
-
-
-
?
3.1.2.27
3-hydroxypalmitoyl-CoA + H2O
substrate is a beta-oxidation intermediate, low activity
646200
Mus musculus
3-hydroxypalmitate + CoA
-
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
-
646200
Mus musculus
chenodeoxycholate + CoA
-
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
best substrate
646200
Mus musculus
chenodeoxycholate + CoA
-
-
-
?
3.1.2.27
choloyl-CoA + H2O
-
646200
Mus musculus
cholate + CoA
-
-
-
?
3.1.2.27
choloyl-CoA + H2O
best substrate
646200
Mus musculus
cholate + CoA
-
-
-
?
3.1.2.27
hydroxymethylglutaryl-CoA + H2O
-
646200
Mus musculus
hydroxymethylglutarate + CoA
-
-
-
?
3.1.2.27
additional information
the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates
646200
Mus musculus
?
-
-
-
-
3.1.2.27
additional information
the enzyme shows broad specificity for substrates and (branched) acyl-CoA chain lengths
646200
Mus musculus
?
-
-
-
-
3.1.2.27
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
trihydroxycoprostanate + CoA
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.27
dimer
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.2
7.4
-
assay at
Mus musculus
3.1.2.20
7.4
-
assay at
Mus musculus
3.1.2.27
7.4
-
assay at
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.2.2
additional information
fasting induces enzyme expression, dependent on peroxisome proliferator-activated receptor alpha
Mus musculus
3.1.2.2
peroxisome proliferator WY-14,643
induction of enzyme expression, dependent on peroxisome proliferator-activated receptor alpha
Mus musculus
3.1.2.2
PPARalpha
i.e. peroxisome proliferator-activated receptor alpha; internal receptor protein essential for effect of stimulating compounds
Mus musculus
3.1.2.20
PPARalpha
i.e. peroxisome proliferator-activated receptor alpha; internal receptor protein essential for effect of stimulating compounds
Mus musculus
3.1.2.27
additional information
fasting induces the enzyme
Mus musculus
3.1.2.27
PPARII
peroxisomal proliferator activated receptor-II activates the enzyme, regulatory function, fasting-mediated induction in vivo, dependent on the peroxisomal proliferator WY-14,643
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.2
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3)
Mus musculus
3.1.2.2
isozyme PTE-2, DNA sequence determination and analysis
Homo sapiens
3.1.2.2
isozyme PTE-2, DNA sequence determination and analysis, expression as green-fluorescent-protein fusion protein in human skin fibroblasts
Mus musculus
3.1.2.20
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3)
Mus musculus
3.1.2.27
gene PTE-2, DNA and amino acid sequence determination and analysis, location on chromosome 20q12-q13, expression in Escherichia coli, expression as GFP-fusion protein in human skin fibroblasts showing targeting to the peroxisomes
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.2
acyl-CoA
substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects
Mus musculus
3.1.2.2
CoA
intraperoxisomal regulatory role
Mus musculus
3.1.2.20
acyl-CoA
substrate inhibition at concentrations higher than 0.005-0.01 mM of acyl-CoA longer than C10, BSA protects
Mus musculus
3.1.2.27
4-chloromercuribenzoate
50% inhibition at 0.001 mM
Mus musculus
3.1.2.27
acyl-CoA
enzyme is substrate-inhibited at 0.005-0.01 mM of acyl-CoAs with chain length longer than C10
Mus musculus
3.1.2.27
CoA
product inhibition with regulatory function, 50% inhibition at 0.01-0.015 mM
Mus musculus
3.1.2.27
DTNB
50% inhibition at 0.15 mM
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.2
additional information
-
additional information
; diverse substrates, kinetics
Mus musculus
3.1.2.2
0.0014
-
palmitoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0017
-
hexadecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0023
-
linoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0025
-
tetradecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0042
-
arachidoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0063
-
trihydroxycoprostanoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0067
-
Arachidonoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0088
-
chenodeoxycholoyl-CoA
pH 7.4
Mus musculus
3.1.2.2
0.0146
-
choloyl-CoA
pH 7.4
Mus musculus
3.1.2.20
additional information
-
additional information
-
Mus musculus
3.1.2.20
0.0014
-
palmitoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0017
-
hexadecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0023
-
linoleoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0025
-
tetradecanoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0042
-
arachidoyl-CoA
pH 7.4
Mus musculus
3.1.2.20
0.0067
-
Arachidonoyl-CoA
pH 7.4
Mus musculus
3.1.2.27
additional information
-
additional information
-
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.2
peroxisome
isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes
Homo sapiens
5777
-
3.1.2.2
peroxisome
isozyme PTE-2, major acyl-CoA thioesterase in peroxisomes; isozyme PTE-II
Mus musculus
5777
-
3.1.2.20
peroxisome
isozyme PTE-II
Mus musculus
5777
-
3.1.2.27
peroxisome
-
Mus musculus
5777
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.27
35886
-
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
3.1.2.27
36000
-
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
3.1.2.27
70000
-
recombinant enzyme, gel filtration
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
Mus musculus
-
CoA + 4,8-dimethylnonanoate
-
-
?
3.1.2.2
additional information
Homo sapiens
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
?
-
Homo sapiens
?
3.1.2.2
additional information
Mus musculus
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
?
-
Mus musculus
?
3.1.2.20
4,8-dimethylnonanoyl-CoA + H2O
Mus musculus
-
CoA + 4,8-dimethylnonanoate
-
-
?
3.1.2.27
2-trans-decenoyl-CoA + H2O
Mus musculus
substrate is a beta-oxidation intermediate, low activity
2-trans-decenoate + CoA
-
-
?
3.1.2.27
3-hydroxypalmitoyl-CoA + H2O
Mus musculus
substrate is a beta-oxidation intermediate, low activity
3-hydroxypalmitate + CoA
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
Mus musculus
-
chenodeoxycholate + CoA
-
-
?
3.1.2.27
choloyl-CoA + H2O
Mus musculus
-
cholate + CoA
-
-
?
3.1.2.27
hydroxymethylglutaryl-CoA + H2O
Mus musculus
-
hydroxymethylglutarate + CoA
-
-
?
3.1.2.27
additional information
Mus musculus
the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates
?
-
-
-
3.1.2.27
trihydroxycoprostanoyl-CoA + H2O
Mus musculus
-
trihydroxycoprostanate + CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.2
recombinant from Escherichia coli
Mus musculus
3.1.2.20
recombinant from Escherichia coli
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.2
heart
-
Mus musculus
-
3.1.2.2
kidney
-
Mus musculus
-
3.1.2.2
liver
-
Mus musculus
-
3.1.2.2
additional information
ubiquitous expression
Mus musculus
-
3.1.2.2
muscle
-
Mus musculus
-
3.1.2.2
testis
-
Mus musculus
-
3.1.2.20
additional information
ubiquitous expression
Mus musculus
-
3.1.2.27
heart
lower enzyme content
Mus musculus
-
3.1.2.27
kidney
high enzyme content
Mus musculus
-
3.1.2.27
liver
-
Mus musculus
-
3.1.2.27
muscle
lower enzyme content
Mus musculus
-
3.1.2.27
testis
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.2
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
-
-
?
3.1.2.2
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
646200
Mus musculus
?
3.1.2.2
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
-
-
?
3.1.2.2
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
646200
Mus musculus
?
3.1.2.2
3-hydroxy-3-methyl glutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methyl glutarate
-
-
-
?
3.1.2.2
3-hydroxy-3-methylglutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methylglutarate
-
646200
Mus musculus
?
3.1.2.2
3-hydroxyhexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxyhexadecanoate
-
-
-
?
3.1.2.2
3-hydroxypalmitoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxypalmitate
-
646200
Mus musculus
?
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
-
-
?
3.1.2.2
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
646200
Mus musculus
?
3.1.2.2
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.2
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
646200
Mus musculus
?
3.1.2.2
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.2
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
646200
Mus musculus
?
3.1.2.2
acyl-CoA + H2O
long-chain fatty-acyl-CoA
646200
Mus musculus
CoA + a carboxylate
-
-
-
?
3.1.2.2
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
-
-
?
3.1.2.2
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
646200
Mus musculus
?
3.1.2.2
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
-
-
?
3.1.2.2
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
646200
Mus musculus
?
3.1.2.2
butyryl-CoA + H2O
-
646200
Mus musculus
butanoate + CoA
-
646200
Mus musculus
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.2
chenodeoxycholoyl-CoA + H2O
-
646200
Mus musculus
CoA + chenodeoxycholate
-
646200
Mus musculus
?
3.1.2.2
choloyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.2
choloyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.2
choloyl-CoA + H2O
best substrate
646200
Mus musculus
CoA + cholate
-
646200
Mus musculus
?
3.1.2.2
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
-
-
?
3.1.2.2
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
646200
Mus musculus
?
3.1.2.2
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
-
-
?
3.1.2.2
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
646200
Mus musculus
?
3.1.2.2
dodecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + dodecanoate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexanoate
-
-
-
?
3.1.2.2
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + n-hexanoate
-
646200
Mus musculus
?
3.1.2.2
lauroyl-CoA + H2O
-
646200
Mus musculus
CoA + laurate
-
646200
Mus musculus
?
3.1.2.2
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
-
-
?
3.1.2.2
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
646200
Mus musculus
?
3.1.2.2
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
-
-
?
3.1.2.2
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
646200
Mus musculus
?
3.1.2.2
additional information
broad chain length specificity
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
additional information
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
646200
Homo sapiens
?
-
646200
Homo sapiens
?
3.1.2.2
additional information
enzyme might play an important role in prevention of CoA sequestration and in facilitating excretion of chain-shortened carboxylic acids, enzyme has a key regulatory function in peroxisomal lipid metabolism
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
myristoleoyl-CoA + H2O
-
646200
Mus musculus
myristoleoate + CoA
-
646200
Mus musculus
?
3.1.2.2
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
-
-
?
3.1.2.2
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
646200
Mus musculus
?
3.1.2.2
n-butyryl-CoA + H2O
-
646200
Mus musculus
CoA + n-butanoate
-
-
-
?
3.1.2.2
octadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octadecanoate
-
-
-
?
3.1.2.2
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
-
-
?
3.1.2.2
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
646200
Mus musculus
?
3.1.2.2
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
-
-
?
3.1.2.2
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
646200
Mus musculus
?
3.1.2.2
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
-
-
?
3.1.2.2
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
646200
Mus musculus
?
3.1.2.2
palmitoyl-CoA + H2O
-
646200
Homo sapiens
CoA + palmitate
-
646200
Homo sapiens
?
3.1.2.2
palmitoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitate
-
646200
Mus musculus
?
3.1.2.2
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
-
-
?
3.1.2.2
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
646200
Mus musculus
?
3.1.2.2
prostaglandin F2alpha + H2O
-
646200
Mus musculus
?
-
646200
Mus musculus
?
3.1.2.2
prostaglandin F2alpha-CoA + H2O
-
646200
Mus musculus
prostaglandin F2alpha + CoA
-
-
-
?
3.1.2.2
stearoyl-CoA + H2O
-
646200
Mus musculus
CoA + stearate
-
646200
Mus musculus
?
3.1.2.2
tetradecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + tetradecanoate
-
-
-
?
3.1.2.2
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
-
-
?
3.1.2.2
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
646200
Mus musculus
?
3.1.2.20
2-methylstearoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-methylstearate
-
-
-
?
3.1.2.20
2-trans-decenoyl-CoA + H2O
-
646200
Mus musculus
CoA + 2-trans-decenoate
-
-
-
?
3.1.2.20
3-hydroxy-3-methyl glutaryl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxy-3-methyl glutarate
-
-
-
?
3.1.2.20
3-hydroxyhexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 3-hydroxyhexadecanoate
-
-
-
?
3.1.2.20
4,8-dimethylnonanoyl-CoA + H2O
-
646200
Mus musculus
CoA + 4,8-dimethylnonanoate
-
-
-
?
3.1.2.20
acetoacetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetoacetate
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
-
646200
Mus musculus
CoA + acetate
-
-
-
?
3.1.2.20
acyl-CoA + H2O
long-chain fatty-acyl-CoA
646200
Mus musculus
CoA + a carboxylate
-
-
-
?
3.1.2.20
arachidonoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidonate
-
-
-
?
3.1.2.20
arachidoyl-CoA + H2O
-
646200
Mus musculus
CoA + arachidate
-
-
-
?
3.1.2.20
chenodeoxycholoyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.20
chenodeoxycholoyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + chenodeoxycholoate
-
-
-
?
3.1.2.20
choloyl-CoA + H2O
activity is PPARalpha-inducible
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.20
choloyl-CoA + H2O
bile acid derivative substrate
646200
Mus musculus
CoA + choloate
-
-
-
?
3.1.2.20
clofibroyl-CoA + H2O
-
646200
Mus musculus
CoA + clofibrate
-
-
-
?
3.1.2.20
decanoyl-CoA + H2O
-
646200
Mus musculus
CoA + decanoate
-
-
-
?
3.1.2.20
dodecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + dodecanoate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
hexanoyl-CoA + H2O
-
646200
Mus musculus
CoA + hexanoate
-
-
-
?
3.1.2.20
linoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + linoleate
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
646200
Mus musculus
CoA + malonate
-
-
-
?
3.1.2.20
myristoyl-CoA + H2O
-
646200
Mus musculus
CoA + myristate
-
-
-
?
3.1.2.20
n-butyryl-CoA + H2O
-
646200
Mus musculus
CoA + n-butanoate
-
-
-
?
3.1.2.20
octadecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octadecanoate
-
-
-
?
3.1.2.20
octanoyl-CoA + H2O
-
646200
Mus musculus
CoA + octanoate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
646200
Mus musculus
CoA + oleate
-
-
-
?
3.1.2.20
palmitoleoyl-CoA + H2O
-
646200
Mus musculus
CoA + palmitoleoate
-
-
-
?
3.1.2.20
propionyl-CoA + H2O
-
646200
Mus musculus
CoA + propionate
-
-
-
?
3.1.2.20
prostaglandin F2alpha-CoA + H2O
-
646200
Mus musculus
prostaglandin F2alpha + CoA
-
-
-
?
3.1.2.20
tetradecanoyl-CoA + H2O
-
646200
Mus musculus
CoA + tetradecanoate
-
-
-
?
3.1.2.20
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
CoA + trihydroxycoprostanoate
-
-
-
?
3.1.2.27
2-trans-decenoyl-CoA + H2O
substrate is a beta-oxidation intermediate, low activity
646200
Mus musculus
2-trans-decenoate + CoA
-
-
-
?
3.1.2.27
3-hydroxypalmitoyl-CoA + H2O
substrate is a beta-oxidation intermediate, low activity
646200
Mus musculus
3-hydroxypalmitate + CoA
-
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
-
646200
Mus musculus
chenodeoxycholate + CoA
-
-
-
?
3.1.2.27
chenodeoxycholoyl-CoA + H2O
best substrate
646200
Mus musculus
chenodeoxycholate + CoA
-
-
-
?
3.1.2.27
choloyl-CoA + H2O
-
646200
Mus musculus
cholate + CoA
-
-
-
?
3.1.2.27
choloyl-CoA + H2O
best substrate
646200
Mus musculus
cholate + CoA
-
-
-
?
3.1.2.27
hydroxymethylglutaryl-CoA + H2O
-
646200
Mus musculus
hydroxymethylglutarate + CoA
-
-
-
?
3.1.2.27
additional information
the enzyme functions as a major regulator of peroxisomal lipid metabolism, the enzyme competes with the bile acid-CoA:amino acid N-acyltranferase for bile acid-CoAs as substrates
646200
Mus musculus
?
-
-
-
-
3.1.2.27
additional information
the enzyme shows broad specificity for substrates and (branched) acyl-CoA chain lengths
646200
Mus musculus
?
-
-
-
-
3.1.2.27
trihydroxycoprostanoyl-CoA + H2O
-
646200
Mus musculus
trihydroxycoprostanate + CoA
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.27
dimer
2 * 35886, amino acid sequence calculation, 2 * 36000, recombinant enzyme, SDS-PAGE
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.2
7.4
-
assay at
Mus musculus
3.1.2.20
7.4
-
assay at
Mus musculus
3.1.2.27
7.4
-
assay at
Mus musculus