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Literature summary extracted from

  • Sabri, A.; Bare, G.; Jacques, P.; Jabrane, A.; Ongena, M.; Van Heugen, J.C.; Devreese, B.; Thonart, P.
    Influence of moderate temperatures on myristoyl-CoA metabolism and acyl-CoA thioesterase activity in the psychrophilic antarctic yeast Rhodotorula aurantiaca (2001), J. Biol. Chem., 276, 12691-12696.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diisopropylfluorophosphate complete inhibition Buckleyzyma aurantiaca
3.1.2.2 myristoyl-CoA substrate inhibition, in vivo 28fold increased substrate concentration at 20°C leads to reduced enzyme activity Buckleyzyma aurantiaca
3.1.2.20 diisopropylfluorophosphate complete inhibition Buckleyzyma aurantiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 additional information
-
 additional information Km at different temperatures Buckleyzyma aurantiaca
3.1.2.2 additional information
-
 additional information Km for myristoyl-CoA is 59fold higher at 20°C compared to 5°C Buckleyzyma aurantiaca
3.1.2.2 0.018
-
 myristoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.018
-
 tetradecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.023
-
 palmitoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.023
-
 hexadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.024
-
 stearoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.024
-
 octadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.058
-
 palmitoleoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.058
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.067
-
 decanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.067
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.118
-
 octanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 0.118
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 additional information
-
 additional information Km at different temperatures Buckleyzyma aurantiaca
3.1.2.20 0.018
-
 tetradecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 0.023
-
 hexadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 0.024
-
 octadecanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 0.058
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 0.067
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 0.118
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 80000
-
 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca
3.1.2.2 85000
-
 gel filtration Buckleyzyma aurantiaca
3.1.2.20 80000
-
 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca
3.1.2.20 85000
-
 gel filtration Buckleyzyma aurantiaca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 additional information Buckleyzyma aurantiaca extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest ?
-
 ?
3.1.2.2 myristoyl-CoA + H2O Buckleyzyma aurantiaca highly preferred substrate, temperature regulated activity, mechanism CoA + myristate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O Buckleyzyma aurantiaca
-
 CoA + tetradecanoate
-
 ?
3.1.2.20 additional information Buckleyzyma aurantiaca extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest ?
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O Buckleyzyma aurantiaca
-
 CoA + tetradecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Buckleyzyma aurantiaca
-
 psychrophilic antarctic yeast, strain A19
-
3.1.2.2 Buckleyzyma aurantiaca
-
 psychrophilic yeast, strain A19
-
3.1.2.20 Buckleyzyma aurantiaca
-
 psychrophilic antarctic yeast, strain A19
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.2.2 glycoprotein highly glycosylated, 19% of the molecular weight is due to carbohydrates Buckleyzyma aurantiaca
3.1.2.2 glycoprotein 19% of the MW is formed by the glycosidic portion Buckleyzyma aurantiaca
3.1.2.20 glycoprotein highly glycosylated, 19% of the molecular weight is due to carbohydrates Buckleyzyma aurantiaca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 351fold Buckleyzyma aurantiaca
3.1.2.20 351fold Buckleyzyma aurantiaca

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.2 palmitoyl-CoA + H2O = CoA + palmitate active site serine residue Buckleyzyma aurantiaca
a
3.1.2.20 an acyl-CoA + H2O = CoA + a carboxylate active site serine residue Buckleyzyma aurantiaca
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 108
-
 purified enzyme Buckleyzyma aurantiaca
3.1.2.2 108
-
 purified enzyme, substrate myristoyl-CoA Buckleyzyma aurantiaca
3.1.2.20 108
-
 purified enzyme, substrate myristoyl-CoA Buckleyzyma aurantiaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + a carboxylate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + decanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Buckleyzyma aurantiaca CoA + hexadecanoate
-
 ?
3.1.2.2 additional information no activity with short-chain acyl-CoA substrates Buckleyzyma aurantiaca ?
-
 ?
3.1.2.2 additional information extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest Buckleyzyma aurantiaca ?
-
 ?
3.1.2.2 myristoyl-CoA + H2O highly preferred substrate Buckleyzyma aurantiaca CoA + myristate
-
 ?
3.1.2.2 myristoyl-CoA + H2O highly preferred substrate, temperature regulated activity, mechanism Buckleyzyma aurantiaca CoA + myristate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Buckleyzyma aurantiaca CoA + octadecanoate
-
 ?
3.1.2.2 octanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + octanoate
-
 ?
3.1.2.2 palmitoleoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + palmitoleoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + palmitate
-
 ?
3.1.2.2 stearoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + stearate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O specific for Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O best substrate Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + a carboxylate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + decanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Buckleyzyma aurantiaca CoA + hexadecanoate
-
 ?
3.1.2.20 additional information extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest Buckleyzyma aurantiaca ?
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Buckleyzyma aurantiaca CoA + octadecanoate
-
 ?
3.1.2.20 octanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + octanoate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + palmitoleoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O
-
 Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O specific for Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O best substrate Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Buckleyzyma aurantiaca CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 monomer 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca
3.1.2.20 monomer 1 * 80000, SDS-PAGE Buckleyzyma aurantiaca

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 long chain acyl-CoA thioesterase
-
 Buckleyzyma aurantiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 5
-
 below, optimal growth temperature in vivo Buckleyzyma aurantiaca
3.1.2.2 45
-
-
 Buckleyzyma aurantiaca
3.1.2.20 45
-
-
 Buckleyzyma aurantiaca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.2.2 additional information
-
 organism is unable to grow at temperatures close to 20°C or above Buckleyzyma aurantiaca
3.1.2.2 additional information
-
 the strain is inable to grow at temperatures close to 20°C and higher Buckleyzyma aurantiaca
3.1.2.20 additional information
-
 organism is unable to grow at temperatures close to 20°C or above Buckleyzyma aurantiaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.2 40
-
 60 min, pH 7.0, 82% remaining activity Buckleyzyma aurantiaca
3.1.2.2 40
-
 60 min, 82% remaining activity Buckleyzyma aurantiaca
3.1.2.2 50
-
 60 min, pH 7.0, 52% remaining activity Buckleyzyma aurantiaca
3.1.2.2 50
-
 60 min, 52% remaining activity Buckleyzyma aurantiaca
3.1.2.2 60
-
 60 min, pH 7.0, 3% remaining activity Buckleyzyma aurantiaca
3.1.2.2 60
-
 60 min, 3% remaining activity Buckleyzyma aurantiaca
3.1.2.20 40
-
 60 min, pH 7.0, 82% remaining activity Buckleyzyma aurantiaca
3.1.2.20 50
-
 60 min, pH 7.0, 52% remaining activity Buckleyzyma aurantiaca
3.1.2.20 60
-
 60 min, pH 7.0, 3% remaining activity Buckleyzyma aurantiaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.2 106
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 106
-
 palmitoleoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 121
-
 stearoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 121
-
 stearoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 136
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 136
-
 decanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 153
-
 palmitoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 153
-
 palmitoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 159
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 159
-
 octanoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.2 183
-
 myristoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.2 183
-
 myristoyl-CoA pH 8.0, 25°C Buckleyzyma aurantiaca
3.1.2.20 106
-
 palmitoleoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 121
-
 stearoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 136
-
 decanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 153
-
 palmitoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 159
-
 octanoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca
3.1.2.20 183
-
 myristoyl-CoA pH 7.4, 25°C Buckleyzyma aurantiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8
-
-
 Buckleyzyma aurantiaca
3.1.2.20 8
-
-
 Buckleyzyma aurantiaca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 5 10
-
 Buckleyzyma aurantiaca
3.1.2.2 5 10 active and stable Buckleyzyma aurantiaca
3.1.2.20 5 10 active and stable Buckleyzyma aurantiaca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.2.2 Buckleyzyma aurantiaca
-
-
 4.4
3.1.2.2 Buckleyzyma aurantiaca isoelectric focusing
-
 4.4
3.1.2.20 Buckleyzyma aurantiaca
-
-
 4.4