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Literature summary extracted from

  • Diczfalusy, M.A.; Alexson, S.E.H.
    Isolation and characterization of novel long-chain acyl-CoA thioesterase/carboxylesterase isoenzymes from Candida rugosa (1996), Arch. Biochem. Biophys., 334, 104-112.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 Triton X-100 slightly stimulates at low concentrations Diutina rugosa
3.1.2.20 Triton X-100 slightly stimulates at low concentrations Diutina rugosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diisopropylfluorophosphate
-
 Diutina rugosa
3.1.2.2 Mg2+
-
 Diutina rugosa
3.1.2.2 p-chloromercuribenzoic acid
-
 Diutina rugosa
3.1.2.2 Triton X-100
-
 Diutina rugosa
3.1.2.20 diisopropylfluorophosphate
-
 Diutina rugosa
3.1.2.20 Mg2+
-
 Diutina rugosa
3.1.2.20 p-chloromercuribenzoic acid
-
 Diutina rugosa
3.1.2.20 Triton X-100
-
 Diutina rugosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.325
-
 dodecanoyl-CoA
-
 Diutina rugosa
3.1.2.20 0.325
-
 dodecanoyl-CoA
-
 Diutina rugosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.2 Ca2+ slightly stimulates at low concentrations Diutina rugosa
3.1.2.2 Mg2+ slightly stimulates at low concentrations Diutina rugosa
3.1.2.20 Ca2+ slightly stimulates at low concentrations Diutina rugosa
3.1.2.20 Mg2+ slightly stimulates at low concentrations Diutina rugosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 40000
-
 gel filtration Diutina rugosa
3.1.2.20 40000
-
 gel filtration Diutina rugosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Diutina rugosa
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Diutina rugosa
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Diutina rugosa
-
-
-
3.1.2.20 Diutina rugosa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 90
-
-
 Diutina rugosa
3.1.2.20 90
-
-
 Diutina rugosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 arachidonoyl-CoA + H2O
-
 Diutina rugosa CoA + arachidonate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Diutina rugosa CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Diutina rugosa CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Diutina rugosa CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Diutina rugosa CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Diutina rugosa CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Diutina rugosa CoA + tetradecanoate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Diutina rugosa CoA + arachidonate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Diutina rugosa CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Diutina rugosa CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Diutina rugosa CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Diutina rugosa CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Diutina rugosa CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Diutina rugosa CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 40000, SDS-PAGE Diutina rugosa
3.1.2.2 monomer 1 * 40000, SDS-PAGE Diutina rugosa
3.1.2.20 ? x * 40000, SDS-PAGE Diutina rugosa
3.1.2.20 monomer 1 * 40000, SDS-PAGE Diutina rugosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 45
-
-
 Diutina rugosa
3.1.2.20 45
-
-
 Diutina rugosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7 7.5
-
 Diutina rugosa
3.1.2.20 7 7.5
-
 Diutina rugosa