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Literature summary extracted from

  • Berge, R.K.; Farstad, M.
    Long-chain fatty acyl-CoA hydrolase from rat liver mitochondria (1981), Methods Enzymol., 71, 234-242.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 bovine serum albumin
-
Rattus norvegicus
3.1.2.20 bovine serum albumin
-
Rattus norvegicus

General Stability

EC Number General Stability Organism
3.1.2.2 glycerol and palmitoyl-CoA stabilize Rattus norvegicus
3.1.2.20 glycerol and palmitoyl-CoA stabilize Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.00013 0.006 fatty-acyl-CoA thioester
-
Rattus norvegicus
3.1.2.2 0.008
-
hexadecanoyl-CoA
-
Rattus norvegicus
3.1.2.20 0.00013 0.006 fatty-acyl-CoA thioester
-
Rattus norvegicus
3.1.2.20 0.008
-
hexadecanoyl-CoA
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 mitochondrion
-
Rattus norvegicus 5739
-
3.1.2.20 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 19000
-
-
Rattus norvegicus
3.1.2.20 19000
-
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Rattus norvegicus
-
CoA + palmitate
-
?
3.1.2.20 palmitoyl-CoA + H2O Rattus norvegicus
-
CoA + palmitate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 706fold Rattus norvegicus
3.1.2.20 706fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 liver
-
Rattus norvegicus
-
3.1.2.20 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 1695
-
-
Rattus norvegicus
3.1.2.20 1695
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Rattus norvegicus
3.1.2.20 -20°C over 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
?
3.1.2.2 oleoyl-CoA + H2O
-
Rattus norvegicus CoA + oleate
-
?
3.1.2.2 palmitoyl-CoA + H2O
-
Rattus norvegicus CoA + palmitate
-
?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
?
3.1.2.20 oleoyl-CoA + H2O
-
Rattus norvegicus CoA + oleate
-
?
3.1.2.20 palmitoyl-CoA + H2O
-
Rattus norvegicus CoA + palmitate
-
?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.5 8
-
Rattus norvegicus
3.1.2.20 7.5 8
-
Rattus norvegicus