Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Riley, D.J.S.; Kyger, E.M.; Spilburg, C.A.; Lange, L.G.
    Pancreatic cholesterol esterases. 2. Purification and characterization of human pancreatic fatty acid ethyl ester synthase (1990), Biochemistry, 29, 3848-3852.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.67 Colipase stimulates hydrolysis of triglycerides, e.g. triolein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.67 NaCl inhibits hydrolysis of triglycerides, e.g. triolein Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.67 cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.67 50000 55000 gel filtration Homo sapiens
3.1.1.67 52000
-
1 * 52000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.67 fatty acid + ethanol Homo sapiens important pathway for the metabolism of ingested ethanol, involved in the metabolism of fatty acids, triglycerides and ethanol fatty acyl ethyl ester + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.67 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.67 1200fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.67 pancreas
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.67 0.04
-
pH 7.2, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.67 arachidonate + ethanol 16.7% of the rate with oleate or linoleate Homo sapiens ethyl arachidonate + H2O
-
?
3.1.1.67 fatty acid + ethanol
-
Homo sapiens fatty acyl ethyl ester + H2O
-
?
3.1.1.67 fatty acid + ethanol important pathway for the metabolism of ingested ethanol, involved in the metabolism of fatty acids, triglycerides and ethanol Homo sapiens fatty acyl ethyl ester + H2O
-
?
3.1.1.67 linoleate + ethanol at the same rate as with oleate Homo sapiens ethyl linoleate + H2O
-
?
3.1.1.67 additional information enzyme has triglyceride lipase activity and also cleaves triglycerides, e.g. triolein, pancreatic fatty acid ethyl ester synthase and triglyceride lipase are likely the same enzymes Homo sapiens ?
-
?
3.1.1.67 additional information enzyme contains a specific heparin-binding site Homo sapiens ?
-
?
3.1.1.67 oleate + ethanol ethyl oleate synthesis to hydrolysis ratio of 80:1, at the same rate as with linoleate Homo sapiens ethyl oleate + H2O
-
r
3.1.1.67 palmitate + ethanol 12.5% of the rate with oleate or linoleate Homo sapiens ethyl palmitate + H2O
-
?
3.1.1.67 stearate + ethanol 4.2% of the rate with oleate or linoleate Homo sapiens ethyl stearate + H2O
-
?
3.1.1.67 triolein + H2O hydrolyzes triglycerides, e.g. triolein, 1.25% of the rate of ethyl oleate or ethyl linoleate synthesis Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.67 monomer 1 * 52000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.67 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.67 7.2
-
assay at Homo sapiens