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Literature summary extracted from

  • Mogelson, S.; Lange, L.G.
    Nonoxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acyl ethyl ester synthase (1984), Biochemistry, 23, 4075-4081.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.67 1-butanol at concentrations greater than 5times Km Oryctolagus cuniculus
3.1.1.67 1-propanol at concentrations greater than 5times Km Oryctolagus cuniculus
3.1.1.67 albumin
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.67 0.2
-
 oleate pH 7.2, 37°C Oryctolagus cuniculus
3.1.1.67 0.2
-
 palmitate pH 7.2, 37°C Oryctolagus cuniculus
3.1.1.67 0.2
-
 linoleate pH 7.2, 37°C Oryctolagus cuniculus
3.1.1.67 0.2
-
 stearate pH 7.2, 37°C Oryctolagus cuniculus
3.1.1.67 0.2
-
 arachidonate pH 7.2, 37°C, presence of ethanol Oryctolagus cuniculus
3.1.1.67 70
-
 1-butanol pH 7.2, 37°C, cosubstrate oleate Oryctolagus cuniculus
3.1.1.67 530
-
 1-propanol pH 7.2, 37°C, cosubstrate oleate Oryctolagus cuniculus
3.1.1.67 1100
-
 ethanol pH 7.2, 37°C, cosubstrate oleate Oryctolagus cuniculus
3.1.1.67 1300
-
 methanol pH 7.2, 37°C, cosubstrate oleate, value above Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.67 soluble
-
 Oryctolagus cuniculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.67 26000
-
 2 * 26000, SDS-PAGE Oryctolagus cuniculus
3.1.1.67 50000
-
 gel filtration under nondenaturing conditions Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.67 fatty acid + ethanol Oryctolagus cuniculus catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP fatty acyl ethyl ester + H2O
-
 ?
3.1.1.67 fatty acid + ethanol Oryctolagus cuniculus production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects fatty acyl ethyl ester + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.67 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.67 5400fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.67 heart ventricular myocardium Oryctolagus cuniculus
-
3.1.1.67 myocardium ventricular Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.67 additional information
-
-
 Oryctolagus cuniculus
3.1.1.67 0.0112
-
 pH 7.2, 37°C Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.67 arachidonate + ethanol 18.3% of the activity with linoleate Oryctolagus cuniculus ethyl arachidonate + H2O
-
 ?
3.1.1.67 fatty acid + ethanol enzyme specificity Oryctolagus cuniculus fatty acyl ethyl ester + H2O
-
 ?
3.1.1.67 fatty acid + ethanol catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP Oryctolagus cuniculus fatty acyl ethyl ester + H2O
-
 ?
3.1.1.67 fatty acid + ethanol production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects Oryctolagus cuniculus fatty acyl ethyl ester + H2O
-
 ?
3.1.1.67 linoleate + ethanol highest activity Oryctolagus cuniculus ethyl linoleate + H2O
-
 ?
3.1.1.67 additional information no hydrolysis of cholesteryl oleate Oryctolagus cuniculus ?
-
 ?
3.1.1.67 oleate + 1-butanol 63.2% of the activity with 1-propanol Oryctolagus cuniculus 1-butyl oleate + H2O
-
 ?
3.1.1.67 oleate + 1-propanol highest activity with 1-propanol Oryctolagus cuniculus 1-propyl oleate + H2O
-
 ?
3.1.1.67 oleate + ethanol 79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol Oryctolagus cuniculus ethyl oleate + H2O
-
 ?
3.1.1.67 oleate + methanol 74.7% of the activity with 1-propanol Oryctolagus cuniculus methyl oleate + H2O
-
 ?
3.1.1.67 palmitate + ethanol 7.3% of the activity with linoleate Oryctolagus cuniculus ethyl palmitate + H2O
-
 ?
3.1.1.67 stearate + ethanol 1.6% of the activity with linoleate Oryctolagus cuniculus ethyl stearate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.67 dimer 2 * 26000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.67 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.67 7.2
-
 assay at Oryctolagus cuniculus