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Literature summary extracted from

  • Anand, G.S.; Goudreau, P.N.; Stock, A.M.
    Activation of methylesterase CheB: evidence of a dual role for the regulatory domain (1998), Biochemistry, 37, 14038-14047.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.61 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.61 A137T 3fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 D56N no activation of enzyme activity in the presence of phosphoramidate Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 H138R 2.5fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 L153P 2fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 R255W 4fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.61 Mg2+ phosphorylation of intact enzyme requires Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.61 additional information Salmonella enterica subsp. enterica serovar Typhimurium demethylation of chemotactic membrane receptors ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.61 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.61 phosphoprotein phosphorylation by phosphoramidate requires Mg2+, phosphorylation of the regulatory domain at Asp-56 results in a reorganization of the domain interface, allowing exposure of the active site to the receptor substrate and simultaneously stimulating methylesterase activity Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.61 protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol mechanism of regulation of enzyme activity by the N-terminal domain Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.61 additional information demethylation of chemotactic membrane receptors Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.61 6
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium