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Literature summary extracted from

  • Kaschabek, S.R.; Kuhn, B.; Muller, D.; Schmidt, E.; Reineke, W.
    Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase (2002), J. Bacteriol., 184, 207-215.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.174 CuSO4 1 mM, 90% inhibition Pseudomonas sp.
2.3.1.174 additional information 1 mM EDTA or 1,10-phenanthroline have no effect Pseudomonas sp.
2.3.1.174 NaCN 1 mM, 12% inhibition Pseudomonas sp.
2.3.1.174 NADH 10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM Pseudomonas sp.
2.3.1.174 ZnCl2 1 mM, 46% inhibition Pseudomonas sp.
2.8.3.6 2-oxoadipate 38% inhibition at 2.5 mM, 58% inhibition at 5 mM Pseudomonas knackmussii
2.8.3.6 NADH 28% inhibition at 0.8 mM Pseudomonas knackmussii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.174 0.01
-
 CoA
-
 Pseudomonas sp.
2.3.1.174 0.15
-
 3-oxoadipyl-CoA
-
 Pseudomonas sp.
2.8.3.6 0.2
-
 succinyl-CoA pH 8, 25°C Pseudomonas knackmussii
2.8.3.6 0.4
-
 3-oxoadipate pH 8, 25°C Pseudomonas knackmussii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.174 42000
-
 4 * 42000, SDS-PAGE Pseudomonas sp.
2.3.1.174 162000
-
 gel filtration Pseudomonas sp.
2.8.3.6 27000
-
 alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE Pseudomonas knackmussii
2.8.3.6 32900
-
 alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE Pseudomonas knackmussii
2.8.3.6 115000
-
 gel filtration Pseudomonas knackmussii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.174 Pseudomonas sp.
-
-
-
2.3.1.174 Pseudomonas sp. B13
-
-
-
2.8.3.6 Pseudomonas knackmussii Q8VPF3 and Q8VPF2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.174
-
 Pseudomonas sp.
2.8.3.6
-
 Pseudomonas knackmussii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.174 11.1
-
-
 Pseudomonas sp.
2.8.3.6 23.9
-
-
 Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.174 CoA + 3-oxoadipyl-CoA
-
 Pseudomonas sp. succinyl-CoA + acetyl-CoA
-
 ?
2.3.1.174 CoA + 3-oxoadipyl-CoA
-
 Pseudomonas sp. B13 succinyl-CoA + acetyl-CoA
-
 ?
2.3.1.174 CoA + oxoadipyl-CoA
-
 Pseudomonas sp. succinyl-CoA + acetyl-CoA
-
 ?
2.3.1.174 CoA + oxoadipyl-CoA
-
 Pseudomonas sp. B13 succinyl-CoA + acetyl-CoA
-
 ?
2.8.3.6 additional information no substrates: 2-chloro-3-oxoadpiate, 2-methyl-3-oxoadipate Pseudomonas knackmussii ?
-
 ?
2.8.3.6 succinyl-CoA + 2-oxoadipate poor substrate Pseudomonas knackmussii succinate + 2-oxoadipyl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 3-oxoadipate
-
 Pseudomonas knackmussii succinate + 3-oxoadipyl-CoA
-
 r
2.8.3.6 succinyl-CoA + 3-oxoglutarate poor substrate Pseudomonas knackmussii succinate + 3-oxoglutaryl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 4-methyl-3-oxoadipate poor substrate Pseudomonas knackmussii succinate + 4-methyl-3-oxoadipyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.174 tetramer 4 * 42000, SDS-PAGE Pseudomonas sp.
2.8.3.6 tetramer alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE Pseudomonas knackmussii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.174 7.8
-
 CoA
-
 Pseudomonas sp.
2.3.1.174 7.8
-
 3-oxoadipyl-CoA
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.174 7.8
-
-
 Pseudomonas sp.
2.8.3.6 8.4
-
-
 Pseudomonas knackmussii