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Literature summary extracted from

  • Sharp, J.A.; Edwards, M.R.
    Purification and properties of succinyl-coenzyme A-3-oxo acid coenzyme A-transferase from sheep kidney (1978), Biochem. J., 173, 759-765.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.5 additional information
-
 amino acid composition Sus scrofa
2.8.3.5 additional information
-
 amino acid composition Ovis aries
2.8.3.5 56000
-
 2 * 56000, SDS-PAGE Ovis aries
2.8.3.5 102000
-
 analytical ultracentrifugation Ovis aries
2.8.3.5 110000
-
 gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Ovis aries
-
-
-
2.8.3.5 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.5
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.5 brain
-
 Sus scrofa
-
2.8.3.5 brain
-
 Ovis aries
-
2.8.3.5 heart
-
 Sus scrofa
-
2.8.3.5 heart
-
 Ovis aries
-
2.8.3.5 kidney
-
 Sus scrofa
-
2.8.3.5 kidney
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.5 200
-
-
 Ovis aries

Storage Stability

EC Number Storage Stability Organism
2.8.3.5 -20°C, at least 1 month Sus scrofa
2.8.3.5 -20°C, at least 1 month Ovis aries
2.8.3.5 storage as ammonium sulfate suspension leads to rapid loss of activity Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate
-
 Sus scrofa succinate + acetoacetyl-CoA
-
 r
2.8.3.5 succinyl-CoA + acetoacetate
-
 Ovis aries succinate + acetoacetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.3.5 dimer
-
 Sus scrofa
2.8.3.5 dimer 2 * 56000, SDS-PAGE Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.5 30
-
 assay at Sus scrofa
2.8.3.5 30
-
 assay at Ovis aries

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.3.5 Sus scrofa pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, brain enzyme
-
 4.8
2.8.3.5 Sus scrofa pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, heart and kidney enzyme
-
 5.5
2.8.3.5 Ovis aries pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, brain enzyme 6.8 6.3
2.8.3.5 Ovis aries pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, heart enzyme
-
 8.2
2.8.3.5 Ovis aries pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, purified kidney enzyme
-
 8.4
2.8.3.5 Ovis aries pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, purified kidney enzyme
-
 8.7
2.8.3.5 Ovis aries pH gradient pH 3.5-10 or pH 7-10 in glycerol gradient, enzyme in kidney extract
-
 9