BRENDA - Enzyme Database

Substrate specificity and mechanism of action of acetoacetate coenzyme A transferase from rat heart

Fenselau, A.; Wallis, K.; Biochemistry 13, 3884-3889 (1974)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.5
2,2-Difluorosuccinate
strong
Rattus norvegicus
2.8.3.5
3-Sulfopropanoate
-
Rattus norvegicus
2.8.3.5
acetoacetate
-
Rattus norvegicus
2.8.3.5
acetylene dicarboxylate
weak
Rattus norvegicus
2.8.3.5
Maleamate
reversible, competitive
Rattus norvegicus
2.8.3.5
Maleimide
succinate or acetoacetate protects
Rattus norvegicus
2.8.3.5
malonate
kinetics
Rattus norvegicus
2.8.3.5
Monomethylsuccinate
competitive
Rattus norvegicus
2.8.3.5
additional information
no inhibition by glutarate, adipate, cis- or trans-cyclobutane-1,2-dicarboxylate, cis- or trans-cyclohexane-1,2-dicarboxylate, methylsuccinate, mercaptosuccinate, malate, aspartate, succinimide or iodoacetamide
Rattus norvegicus
2.8.3.5
N-ethylmaleamate
reversible, competitive
Rattus norvegicus
2.8.3.5
N-ethylmaleimide
succinate or acetoacetate protects
Rattus norvegicus
2.8.3.5
oxalate
kinetics
Rattus norvegicus
2.8.3.5
Perfluorosuccinate
strong
Rattus norvegicus
2.8.3.5
succinamate
competitive
Rattus norvegicus
2.8.3.5
succinate
product inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.5
0.04
-
acetoacetyl-CoA
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
0.28
-
succinyl-CoA
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
0.44
-
acetoacetate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
28
-
succinate
cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
35
-
Maleate
cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.3.5
mitochondrion
-
Rattus norvegicus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.3.5
Rattus norvegicus
-
male adult Buffalo
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.3.5
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.5
heart
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.5
2.88
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.8.3.5
frozen, partially purified preparation in phosphate solution, less than 10% loss of activity within 1-2 weeks
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.5
additional information
no substrates are diacids with connecting chain lengths of 3 or more methylene groups
645978
Rattus norvegicus
?
-
-
-
-
2.8.3.5
succinyl-CoA + acetoacetate
-
645978
Rattus norvegicus
succinate + acetoacetyl-CoA
-
645978
Rattus norvegicus
r
2.8.3.5
succinyl-CoA + maleate
-
645978
Rattus norvegicus
succinate + maleyl-CoA
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
assay at
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.5
0.72
-
succinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
3.7
-
acetoacetate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
6.4
-
2,2-Difluorosuccinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
15
-
oxalate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
18
-
Perfluorosuccinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
21
-
malonate
pH 8.1, 25°C
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.5
2,2-Difluorosuccinate
strong
Rattus norvegicus
2.8.3.5
3-Sulfopropanoate
-
Rattus norvegicus
2.8.3.5
acetoacetate
-
Rattus norvegicus
2.8.3.5
acetylene dicarboxylate
weak
Rattus norvegicus
2.8.3.5
Maleamate
reversible, competitive
Rattus norvegicus
2.8.3.5
Maleimide
succinate or acetoacetate protects
Rattus norvegicus
2.8.3.5
malonate
kinetics
Rattus norvegicus
2.8.3.5
Monomethylsuccinate
competitive
Rattus norvegicus
2.8.3.5
additional information
no inhibition by glutarate, adipate, cis- or trans-cyclobutane-1,2-dicarboxylate, cis- or trans-cyclohexane-1,2-dicarboxylate, methylsuccinate, mercaptosuccinate, malate, aspartate, succinimide or iodoacetamide
Rattus norvegicus
2.8.3.5
N-ethylmaleamate
reversible, competitive
Rattus norvegicus
2.8.3.5
N-ethylmaleimide
succinate or acetoacetate protects
Rattus norvegicus
2.8.3.5
oxalate
kinetics
Rattus norvegicus
2.8.3.5
Perfluorosuccinate
strong
Rattus norvegicus
2.8.3.5
succinamate
competitive
Rattus norvegicus
2.8.3.5
succinate
product inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.5
0.72
-
succinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
3.7
-
acetoacetate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
6.4
-
2,2-Difluorosuccinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
15
-
oxalate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
18
-
Perfluorosuccinate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
21
-
malonate
pH 8.1, 25°C
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.5
0.04
-
acetoacetyl-CoA
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
0.28
-
succinyl-CoA
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
0.44
-
acetoacetate
pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
28
-
succinate
cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C
Rattus norvegicus
2.8.3.5
35
-
Maleate
cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.3.5
mitochondrion
-
Rattus norvegicus
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.3.5
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.5
heart
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.5
2.88
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.3.5
frozen, partially purified preparation in phosphate solution, less than 10% loss of activity within 1-2 weeks
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.5
additional information
no substrates are diacids with connecting chain lengths of 3 or more methylene groups
645978
Rattus norvegicus
?
-
-
-
-
2.8.3.5
succinyl-CoA + acetoacetate
-
645978
Rattus norvegicus
succinate + acetoacetyl-CoA
-
645978
Rattus norvegicus
r
2.8.3.5
succinyl-CoA + maleate
-
645978
Rattus norvegicus
succinate + maleyl-CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
assay at
Rattus norvegicus