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Literature summary extracted from

  • Hersh, L.B.; Jencks, W.P.
    Coenzyme A transferase. Kinetics and exchange reactions (1967), J. Biol. Chem., 242, 3468-3480.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.5 2,4-Dinitrophenylacetate at pH 7.9, less inactivating activity at pH 7, acetoacetyl-CoA protects Sus scrofa
2.8.3.5 4-nitrophenylacetate at pH 7.9, less inactivating activity at pH 7 Sus scrofa
2.8.3.5 Acetic anhydride
-
 Sus scrofa
2.8.3.5 acetoacetate kinetics; product inhibition Sus scrofa
2.8.3.5 acetoacetyl-CoA EDTA, trisodium citrate and diphosphate protect, too, addition of Cu2+, Mn2+, Ca2+ or Zn2+ (decreasing order) restores inactivating activity of acetoacetyl-CoA; in the absence of succinate, cysteine restores, succinate or 0.1 M NaCl protects Sus scrofa
2.8.3.5 Acetylimidazole equally efficient at pH 7 and 7.9 Sus scrofa
2.8.3.5 citrate 0.167 M, weak Sus scrofa
2.8.3.5 Glutarate 0.1 M, weak Sus scrofa
2.8.3.5 HPO42- 0.1 M, weak Sus scrofa
2.8.3.5 malate 0.1 M, weak Sus scrofa
2.8.3.5 malonate 0.1 M, weak Sus scrofa
2.8.3.5 Monovalent anions decreasing order of effectiveness: SCN-, ClO4-, I-, Br-, Cl-, not F- Sus scrofa
2.8.3.5 NaCl kinetics, 24% inhibition at 10 mM Sus scrofa
2.8.3.5 NaI 57% inhibition at 10 mM Sus scrofa
2.8.3.5 SCN- 47% inhibition at 10 mM Sus scrofa
2.8.3.5 SO42- 0.1 M, weak Sus scrofa
2.8.3.5 succinate kinetics; product inhibition Sus scrofa
2.8.3.5 succinyl-CoA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.5 additional information
-
 additional information kinetic study Sus scrofa
2.8.3.5 0.2
-
 acetoacetate cosusbstrate: succinyl-CoA, pH 8.1 Sus scrofa
2.8.3.5 0.72
-
 acetoacetyl-CoA cosusbstrate: succinate, pH 8.1 Sus scrofa
2.8.3.5 4.2
-
 succinyl-CoA cosusbstrate: acetoacetate, pH 8.1 Sus scrofa
2.8.3.5 36
-
 succinate cosusbstrate: acetoacetyl-CoA, pH 8.1 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.5 78000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.5 partial Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.5 succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.5 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.5 3
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.8.3.5 -20°C, 1.4 mg protein/ml, 0.02 M potassium phosphate buffer, pH 7.4, t1/2: 9 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 additional information catalyzes exchange reactions in the absence of cosubstrates: succinate/succinyl-CoA and acetoacetate/acetoacetyl-CoA Sus scrofa ?
-
 ?
2.8.3.5 succinyl-CoA + acetoacetate
-
 Sus scrofa succinate + acetoacetyl-CoA
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.5 25
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.3.5 25
-
 1 min, inactivation at pH-values below pH 3.1 or in 0.1 M NaOH, slow loss of activity at pH 5 and pH 10.7 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.5 37.3
-
 succinyl-CoA pH 8.1 Sus scrofa
2.8.3.5 933
-
 acetoacetyl-CoA pH 8.1 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.5 8 8.7
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.3.5 7.1 8.7 about 50% of activity at pH 7.1, optimum at pH 8-8.7 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.3.5 3.1
-
 below, 1 min at 25°C, inactivation Sus scrofa
2.8.3.5 5
-
 slow loss of activity at 25°C Sus scrofa
2.8.3.5 10.7
-
 slow loss of activity at 25°C Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.5 0.13
-
 acetoacetyl CoA pH 8.1 Sus scrofa
2.8.3.5 0.78
-
 acetoacetate pH 8.1 Sus scrofa
2.8.3.5 1
-
 succinate pH 8.1 Sus scrofa
2.8.3.5 1.9
-
 succinyl-CoA pH 8.1 Sus scrofa