BRENDA - Enzyme Database

Coenzyme A transferase. Kinetics and exchange reactions

Hersh, L.B.; Jencks, W.P.; J. Biol. Chem. 242, 3468-3480 (1967)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.5
2,4-Dinitrophenylacetate
at pH 7.9, less inactivating activity at pH 7, acetoacetyl-CoA protects
Sus scrofa
2.8.3.5
4-nitrophenylacetate
at pH 7.9, less inactivating activity at pH 7
Sus scrofa
2.8.3.5
Acetic anhydride
-
Sus scrofa
2.8.3.5
acetoacetate
kinetics; product inhibition
Sus scrofa
2.8.3.5
acetoacetyl-CoA
EDTA, trisodium citrate and diphosphate protect, too, addition of Cu2+, Mn2+, Ca2+ or Zn2+ (decreasing order) restores inactivating activity of acetoacetyl-CoA; in the absence of succinate, cysteine restores, succinate or 0.1 M NaCl protects
Sus scrofa
2.8.3.5
Acetylimidazole
equally efficient at pH 7 and 7.9
Sus scrofa
2.8.3.5
citrate
0.167 M, weak
Sus scrofa
2.8.3.5
Glutarate
0.1 M, weak
Sus scrofa
2.8.3.5
HPO42-
0.1 M, weak
Sus scrofa
2.8.3.5
malate
0.1 M, weak
Sus scrofa
2.8.3.5
malonate
0.1 M, weak
Sus scrofa
2.8.3.5
Monovalent anions
decreasing order of effectiveness: SCN-, ClO4-, I-, Br-, Cl-, not F-
Sus scrofa
2.8.3.5
NaCl
kinetics, 24% inhibition at 10 mM
Sus scrofa
2.8.3.5
NaI
57% inhibition at 10 mM
Sus scrofa
2.8.3.5
SCN-
47% inhibition at 10 mM
Sus scrofa
2.8.3.5
SO42-
0.1 M, weak
Sus scrofa
2.8.3.5
succinate
kinetics; product inhibition
Sus scrofa
2.8.3.5
succinyl-CoA
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.5
additional information
-
additional information
kinetic study
Sus scrofa
2.8.3.5
0.2
-
acetoacetate
cosusbstrate: succinyl-CoA, pH 8.1
Sus scrofa
2.8.3.5
0.72
-
acetoacetyl-CoA
cosusbstrate: succinate, pH 8.1
Sus scrofa
2.8.3.5
4.2
-
succinyl-CoA
cosusbstrate: acetoacetate, pH 8.1
Sus scrofa
2.8.3.5
36
-
succinate
cosusbstrate: acetoacetyl-CoA, pH 8.1
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.5
78000
-
gel filtration
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.3.5
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.3.5
partial
Sus scrofa
Reaction
EC Number
Reaction
Commentary
Organism
2.8.3.5
succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA
mechanism
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.5
heart
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.5
3
-
-
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.8.3.5
-20°C, 1.4 mg protein/ml, 0.02 M potassium phosphate buffer, pH 7.4, t1/2: 9 months
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.5
additional information
catalyzes exchange reactions in the absence of cosubstrates: succinate/succinyl-CoA and acetoacetate/acetoacetyl-CoA
645967
Sus scrofa
?
-
-
-
-
2.8.3.5
succinyl-CoA + acetoacetate
-
645967
Sus scrofa
succinate + acetoacetyl-CoA
-
645967
Sus scrofa
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
assay at
Sus scrofa
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
1 min, inactivation at pH-values below pH 3.1 or in 0.1 M NaOH, slow loss of activity at pH 5 and pH 10.7
Sus scrofa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8.3.5
37.3
-
succinyl-CoA
pH 8.1
Sus scrofa
2.8.3.5
933
-
acetoacetyl-CoA
pH 8.1
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.3.5
8
8.7
-
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.8.3.5
7.1
8.7
about 50% of activity at pH 7.1, optimum at pH 8-8.7
Sus scrofa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.8.3.5
3.1
-
below, 1 min at 25°C, inactivation
Sus scrofa
2.8.3.5
5
-
slow loss of activity at 25°C
Sus scrofa
2.8.3.5
10.7
-
slow loss of activity at 25°C
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.5
0.13
-
acetoacetyl CoA
pH 8.1
Sus scrofa
2.8.3.5
0.78
-
acetoacetate
pH 8.1
Sus scrofa
2.8.3.5
1
-
succinate
pH 8.1
Sus scrofa
2.8.3.5
1.9
-
succinyl-CoA
pH 8.1
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.3.5
2,4-Dinitrophenylacetate
at pH 7.9, less inactivating activity at pH 7, acetoacetyl-CoA protects
Sus scrofa
2.8.3.5
4-nitrophenylacetate
at pH 7.9, less inactivating activity at pH 7
Sus scrofa
2.8.3.5
Acetic anhydride
-
Sus scrofa
2.8.3.5
acetoacetate
kinetics; product inhibition
Sus scrofa
2.8.3.5
acetoacetyl-CoA
EDTA, trisodium citrate and diphosphate protect, too, addition of Cu2+, Mn2+, Ca2+ or Zn2+ (decreasing order) restores inactivating activity of acetoacetyl-CoA; in the absence of succinate, cysteine restores, succinate or 0.1 M NaCl protects
Sus scrofa
2.8.3.5
Acetylimidazole
equally efficient at pH 7 and 7.9
Sus scrofa
2.8.3.5
citrate
0.167 M, weak
Sus scrofa
2.8.3.5
Glutarate
0.1 M, weak
Sus scrofa
2.8.3.5
HPO42-
0.1 M, weak
Sus scrofa
2.8.3.5
malate
0.1 M, weak
Sus scrofa
2.8.3.5
malonate
0.1 M, weak
Sus scrofa
2.8.3.5
Monovalent anions
decreasing order of effectiveness: SCN-, ClO4-, I-, Br-, Cl-, not F-
Sus scrofa
2.8.3.5
NaCl
kinetics, 24% inhibition at 10 mM
Sus scrofa
2.8.3.5
NaI
57% inhibition at 10 mM
Sus scrofa
2.8.3.5
SCN-
47% inhibition at 10 mM
Sus scrofa
2.8.3.5
SO42-
0.1 M, weak
Sus scrofa
2.8.3.5
succinate
kinetics; product inhibition
Sus scrofa
2.8.3.5
succinyl-CoA
-
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.8.3.5
0.13
-
acetoacetyl CoA
pH 8.1
Sus scrofa
2.8.3.5
0.78
-
acetoacetate
pH 8.1
Sus scrofa
2.8.3.5
1
-
succinate
pH 8.1
Sus scrofa
2.8.3.5
1.9
-
succinyl-CoA
pH 8.1
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.3.5
additional information
-
additional information
kinetic study
Sus scrofa
2.8.3.5
0.2
-
acetoacetate
cosusbstrate: succinyl-CoA, pH 8.1
Sus scrofa
2.8.3.5
0.72
-
acetoacetyl-CoA
cosusbstrate: succinate, pH 8.1
Sus scrofa
2.8.3.5
4.2
-
succinyl-CoA
cosusbstrate: acetoacetate, pH 8.1
Sus scrofa
2.8.3.5
36
-
succinate
cosusbstrate: acetoacetyl-CoA, pH 8.1
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.3.5
78000
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.3.5
partial
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.3.5
heart
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.8.3.5
3
-
-
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.8.3.5
-20°C, 1.4 mg protein/ml, 0.02 M potassium phosphate buffer, pH 7.4, t1/2: 9 months
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.3.5
additional information
catalyzes exchange reactions in the absence of cosubstrates: succinate/succinyl-CoA and acetoacetate/acetoacetyl-CoA
645967
Sus scrofa
?
-
-
-
-
2.8.3.5
succinyl-CoA + acetoacetate
-
645967
Sus scrofa
succinate + acetoacetyl-CoA
-
645967
Sus scrofa
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
assay at
Sus scrofa
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.3.5
25
-
1 min, inactivation at pH-values below pH 3.1 or in 0.1 M NaOH, slow loss of activity at pH 5 and pH 10.7
Sus scrofa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8.3.5
37.3
-
succinyl-CoA
pH 8.1
Sus scrofa
2.8.3.5
933
-
acetoacetyl-CoA
pH 8.1
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8.3.5
8
8.7
-
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.8.3.5
7.1
8.7
about 50% of activity at pH 7.1, optimum at pH 8-8.7
Sus scrofa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.8.3.5
3.1
-
below, 1 min at 25°C, inactivation
Sus scrofa
2.8.3.5
5
-
slow loss of activity at 25°C
Sus scrofa
2.8.3.5
10.7
-
slow loss of activity at 25°C
Sus scrofa