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Literature summary extracted from

  • Sidhu, H.; Ogden, S.D.; Lung, H.Y.; Luttge, B.G.; Baetz, A.L.; Peck, A.B.
    DNA sequencing and expression of the formyl coenzyme A transferase gene, frc, from, Oxalobacter formigenes (1997), J. Bacteriol., 179, 3378-3381.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.16 DNA sequence determination and analysis, monocistronic operon, overexpression in Escherichia coli, activity is similar to the wild-type enzyme Oxalobacter formigenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.16 45000
-
 1 * 45000, recombinant enzyme, SDS-PAGE Oxalobacter formigenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate Oxalobacter formigenes activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora formate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.16 Oxalobacter formigenes O06644 gene frc
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.16 recombinant from Escherichia coli JM109 Oxalobacter formigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate
-
 Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
2.8.3.16 formyl-CoA + oxalate activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
2.8.3.16 succinyl-CoA + oxalate no activity with the recombinant enzyme expressed in Escherichia coli Oxalobacter formigenes succinate + oxalyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.16 monomer 1 * 45000, recombinant enzyme, SDS-PAGE Oxalobacter formigenes