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Literature summary extracted from

  • Baetz, A.L.; Allison, M.J.
    Purification and characterization of formyl-coenzyme A transferase from Oxalobacter formigenes (1990), J. Bacteriol., 172, 3537-3540.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.16 N-ethylmaleimide 20% inhibition at 1 mM Oxalobacter formigenes
2.8.3.16 p-chloromercuribenzoate 91% inhibition at 1 mM, causes precipitation Oxalobacter formigenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.16 2.3
-
 succinate pH 6.7, 25°C Oxalobacter formigenes
2.8.3.16 3
-
 formyl-CoA pH 6.7, 25°C Oxalobacter formigenes
2.8.3.16 5.1
-
 oxalate pH 6.7, 25°C Oxalobacter formigenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.16 44000
-
 1 * 44000, SDS-PAGE Oxalobacter formigenes
2.8.3.16 44700
-
 gel filtration Oxalobacter formigenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate Oxalobacter formigenes
-
 formate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.16 Oxalobacter formigenes O06644
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.16 31.2fold, to homogeneity Oxalobacter formigenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.16 2.15
-
 purified enzyme Oxalobacter formigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate
-
 Oxalobacter formigenes formate + oxalyl-CoA
-
 ?
2.8.3.16 additional information no activity with acetate or malonate Oxalobacter formigenes ?
-
 ?
2.8.3.16 succinyl-CoA + oxalate 11% of the reaction rate observed with formyl-CoA Oxalobacter formigenes succinate + oxalyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.16 monomer 1 * 44000, SDS-PAGE Oxalobacter formigenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.16 25
-
 assay at Oxalobacter formigenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.16 6.5 7.5
-
 Oxalobacter formigenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.3.16 Oxalobacter formigenes
-
 4.8 4.7