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Literature summary extracted from

  • Seo, H.S.; Song, J.T.; Cheong, J.J.; Lee, Y.H.; Lee, Y.W.; Hwang, I.; Lee, J.S.; Choi, Y.D.
    Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses (2001), Proc. Natl. Acad. Sci. USA, 98, 4788-4793.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.141 expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.141 Ca2+ 5 mM, 80% loss of activity Arabidopsis thaliana
2.1.1.141 Cu2+ 5 mM, 80% loss of activity Arabidopsis thaliana
2.1.1.141 Fe2+ 5 mM, 80% loss of activity Arabidopsis thaliana
2.1.1.141 Mg2+ 5 mM, 80% loss of activity Arabidopsis thaliana
2.1.1.141 Mn2+ 5 mM, 80% loss of activity Arabidopsis thaliana
2.1.1.141 Na+ 5 mM, 50-60% inhibition Arabidopsis thaliana
2.1.1.141 NH4+ 5 mM, 50-60% inhibition Arabidopsis thaliana
2.1.1.141 Zn2+ 5 mM, 80% loss of activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.141 0.0063
-
 S-adenosyl-L-methionine pH 7.5, 20°C Arabidopsis thaliana
2.1.1.141 0.0385
-
 jasmonate pH 7.5, 20°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.141 cytoplasm
-
 Arabidopsis thaliana 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.141 KCl highest activity in presence of 100 mM KCl Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.141 S-adenosyl-L-methionine + jasmonate Arabidopsis thaliana expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.141 Arabidopsis thaliana Q9AR07
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.141
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.141 additional information the enzyme does not convert 12-oxo-phytodienoic acid, a precursor of jasmonic acid, salicylic acid, benzoic acid, linolenic acid or cinnamic acid into their corresponding methyl esters Arabidopsis thaliana ?
-
 ?
2.1.1.141 S-adenosyl-L-methionine + 9,10-dihydrojasmonic acid poor substrate Arabidopsis thaliana S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.141 S-adenosyl-L-methionine + jasmonate
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?
2.1.1.141 S-adenosyl-L-methionine + jasmonate expression of the gene is induced both locally and systematically by wounding or methyl jasmonate treatement. The jasmonic acid carboxyl methyltransferase is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate that could act as an intracellular regulator, a diffusible intercellular transducer, and an airborne signal mediating intra- and interplant communications Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.141 20
-
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.141 25
-
 jasmonate pH 7.5, 20°C Arabidopsis thaliana
2.1.1.141 70
-
 S-adenosyl-L-methionine pH 7.5, 20°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.141 7 8
-
 Arabidopsis thaliana