Literature summary extracted from

Otake, Y.; Nolan, A.L.; Walle, U.K.; Walle, T.
Quercetin and resveratrol potently reduce estrogen sulfotransferase activity in normal human mammary epithelial cells (2000), J. Steroid Biochem. Mol. Biol., 73, 265-270.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.8.2.4	EST cDNA subcloned into pKK233-2 and expressed in Escherichia coli XL1-Blue	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.8.2.4	quercetin	competitive inhibition	Homo sapiens
2.8.2.4	resveratrol	competitive inhibition	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8.2.4	0.00032	-	quercetin	pH 7.4, 37°C	Homo sapiens
2.8.2.4	0.00058	-	resveratrol	pH 7.4, 37°C	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.2.4	Homo sapiens	-	human	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.8.2.4	recombinant enzyme	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.8.2.4	epithelium	mammary epithelial cells	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.2.4	3'-phosphoadenylylsulfate + 17beta-estradiol	-	Homo sapiens	adenosine 3',5'-bisphosphate + estradiol 3-sulfate	-	r
2.8.2.4	3'-phosphoadenylylsulfate + quercetin	-	Homo sapiens	adenosine 3',5'-bisphosphate + quercetin sulfate	-	r
2.8.2.4	3'-phosphoadenylylsulfate + resveratrol	-	Homo sapiens	adenosine 3',5'-bisphosphate + resveratrol sulfate	-	r

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.8.2.4	0.00036	-	resveratrol	pH 7.4, 37°C	Homo sapiens
2.8.2.4	0.00058	-	quercetin	pH 7.4, 37°C	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)