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Literature summary extracted from

  • Hempel, N.; Barnett, A.C.; Bolton-Grob, R.M.; Liyou, N.E.; McManus, M.E.
    Site-directed mutagenesis of the substrate-binding cleft of human estrogen sulfotransferase (2000), Biochem. Biophys. Res. Commun., 276, 224-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.4
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.4 H107A site-directed mutagenesis, humSULT1E1, presence of a histidine residue at position 107 is essential for the production of a functional protein, substitution of this amino acid to alanine results in complete loss of activity towards all substrates tested Homo sapiens
2.8.2.4 K85A site-directed mutagenesis, humSULT1E1 Homo sapiens
2.8.2.4 V145E site-directed mutagenesis, humSULT1E1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.4 0.00005
-
 dehydroepiandrosterone pH 7.0, 37°C, SULT1E1-K85A Homo sapiens
2.8.2.4 0.0001
-
 estrone pH 7.0, 37°C, SULT1E1-K85A Homo sapiens
2.8.2.4 0.0001
-
 17beta-estradiol pH 7.0, 37°C, SULT1E1-K85A Homo sapiens
2.8.2.4 0.0002
-
 dehydroepiandrosterone pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.0002
-
 estrone pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.0003
-
 17beta-estradiol pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.00046
-
 3'-phosphoadenylylsulfate pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.00069
-
 3'-phosphoadenylylsulfate pH 7.0, 37°C, mutant SULT1E1-K85A Homo sapiens
2.8.2.4 0.0036
-
 estrone pH 7.0, 37°C, SULT1E1-V145E Homo sapiens
2.8.2.4 0.0082
-
 17beta-estradiol pH 7.0, 37°C, SULT1E1-V145E Homo sapiens
2.8.2.4 0.01318
-
 3'-phosphoadenylylsulfate pH 7.0, 37°C, mutant SULT1E1-V145E Homo sapiens
2.8.2.4 0.0607
-
 dehydroepiandrosterone pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.0766
-
 p-nitrophenol pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 0.2407
-
 dopamine pH 7.0, 37°C, SULT1E1-V145E Homo sapiens
2.8.2.4 0.6248
-
 p-nitrophenol pH 7.0, 37°C, SULT1E1-K85A Homo sapiens
2.8.2.4 0.9372
-
 p-nitrophenol pH 7.0, 37°C, SULT1E1-V145E Homo sapiens
2.8.2.4 32.22
-
 dopamine pH 7.0, 37°C, SULT1E1-wild-type Homo sapiens
2.8.2.4 79.35
-
 dopamine pH 7.0, 37°C, SULT1E1-K85A Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.4 35120
-
 calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estrone Homo sapiens
-
 adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.4 Homo sapiens
-
 human
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + 17beta-estradiol
-
 Homo sapiens adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + dopamine
-
 Homo sapiens adenosine 3',5'-bisphosphate + dopamine sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone
-
 Homo sapiens adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + p-nitrophenol model substrate, sulfonated by SULT1E1 Homo sapiens adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.8.2.4 SULT1E1
-
 Homo sapiens