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Literature summary extracted from

  • Adams, J.B.; Ellyard, R.K.; Low, J.
    Enzymic synthesis of steroid sulphates. IX. Physical and chemical properties of purified oestrogen sulphotransferase from bovine adrenal glands, the nature of its isoenzymic forms and a proposed model to explain its wave-like kinetics (1974), Biochim. Biophys. Acta, 370, 160-188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.4 2-mercaptoethanol activation Bos taurus
2.8.2.4 cysteine
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.4 3'-AMP
-
 Bos taurus
2.8.2.4 3'-phosphoadenylylsulfate competitive inhibition Bos taurus
2.8.2.4 ADP non-competitive inhibitor Bos taurus
2.8.2.4 ATP mixed-type inhibition Bos taurus
2.8.2.4 p-hydroxymercuribenzoate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.4 0.015
-
 estrone pH 8.1, 37°C Bos taurus
2.8.2.4 0.037
-
 3'-phosphoadenylylsulfate pH 8.1, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.4 73800
-
 sedimentation equilibrium centrifugation Bos taurus
2.8.2.4 74000
-
 sucrose density gradient, pH 5.8 Bos taurus
2.8.2.4 74800
-
 meniscus depletion method, sucrose density gradient centrifugation, pH 7.0 Bos taurus
2.8.2.4 76000
-
 sucrose density gradient centrifugation, pH 9.0 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estrone Bos taurus
-
 adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.4 adrenal enzyme Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.4 adrenal gland
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + 17beta-estradiol
-
 Bos taurus adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
 r
2.8.2.4 3'-phosphoadenylylsulfate + estrone
-
 Bos taurus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.2.4 monomer 1 * 74000, SDS-PAGE Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.4 0.056
-
 3'-phosphoadenylylsulfate pH 8.1, 37°C Bos taurus
2.8.2.4 1.9
-
 3'-AMP pH 8.1, 37°C Bos taurus
2.8.2.4 2.8
-
 ADP pH 8.1, 37°C Bos taurus