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Literature summary extracted from

  • Rens-Domiano, S.; Roth, J.A.
    Characterization of tyrosylprotein sulfotransferase from rat liver and other tissues (1989), J. Biol. Chem., 264, 899-905.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.20 5'-AMP increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate Rattus norvegicus
2.8.2.20 CHAPS activation, 0.5% w/v Rattus norvegicus
2.8.2.20 Lubrol Px activation Homo sapiens
2.8.2.20 Lubrol Px activation Rattus norvegicus
2.8.2.20 Lubrol Px 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v Homo sapiens
2.8.2.20 Lubrol Px 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v Rattus norvegicus
2.8.2.20 additional information no activation by octyl glucoside Rattus norvegicus
2.8.2.20 Nonidet P-40 activation, 0.5% w/v Rattus norvegicus
2.8.2.20 Triton X-100 activation Homo sapiens
2.8.2.20 Triton X-100 activation Rattus norvegicus
2.8.2.20 Tween 20 activation, 0.5% w/v Rattus norvegicus
2.8.2.20 Tween 80 activation, 0.5% w/v Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.20 Lubrol Px 3fold stimulation at 0.1% w/v, weak inhibition above 0.1% w/v Rattus norvegicus
2.8.2.20 Mn2+ above 30 mM, activation below Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi membrane
-
 Mus musculus 139
-
2.8.2.20 Golgi membrane
-
 Homo sapiens 139
-
2.8.2.20 Golgi membrane
-
 Rattus norvegicus 139
-
2.8.2.20 Golgi membrane
-
 Oryctolagus cuniculus 139
-
2.8.2.20 Golgi membrane
-
 Mesocricetus auratus 139
-
2.8.2.20 membrane
-
 Mus musculus 16020
-
2.8.2.20 membrane
-
 Homo sapiens 16020
-
2.8.2.20 membrane
-
 Rattus norvegicus 16020
-
2.8.2.20 membrane
-
 Oryctolagus cuniculus 16020
-
2.8.2.20 membrane
-
 Mesocricetus auratus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Co2+ 25% of the stimulation with Mn2+, 20 mM Rattus norvegicus
2.8.2.20 Mn2+ requirement Homo sapiens
2.8.2.20 Mn2+ requirement Rattus norvegicus
2.8.2.20 Mn2+ 20 mM Rattus norvegicus
2.8.2.20 Mn2+ inhibits above 30 mM Rattus norvegicus
2.8.2.20 additional information no requirement of Mg2+ Rattus norvegicus
2.8.2.20 additional information no activation by Ca2+, Cd2+, Cu2+ or Zn2+ Rattus norvegicus
2.8.2.20 NaF increases product formation 10fold by preventing degradation of 3'-phosphoadenylylsulfate Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Mus musculus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Homo sapiens post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Rattus norvegicus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Oryctolagus cuniculus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine Mesocricetus auratus post-translational modification of biologically active peptides and proteins adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Homo sapiens
-
-
-
2.8.2.20 Mesocricetus auratus
-
-
-
2.8.2.20 Mus musculus
-
-
-
2.8.2.20 Oryctolagus cuniculus
-
-
-
2.8.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.20 liver Rattus norvegicus
2.8.2.20 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.20 brain
-
 Rattus norvegicus
-
2.8.2.20 cerebellum
-
 Rattus norvegicus
-
2.8.2.20 liver
-
 Mus musculus
-
2.8.2.20 liver
-
 Homo sapiens
-
2.8.2.20 liver
-
 Rattus norvegicus
-
2.8.2.20 liver
-
 Oryctolagus cuniculus
-
2.8.2.20 liver
-
 Mesocricetus auratus
-
2.8.2.20 lung
-
 Rattus norvegicus
-
2.8.2.20 additional information tissue distribution Rattus norvegicus
-
2.8.2.20 pituitary gland
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.8.2.20 -80°C, in 25% glycerol, 30% loss of activity within 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Mus musculus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Oryctolagus cuniculus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Mesocricetus auratus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Mus musculus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Homo sapiens adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Rattus norvegicus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Oryctolagus cuniculus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + protein tyrosine post-translational modification of biologically active peptides and proteins Mesocricetus auratus adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.20 30
-
 assay at Mus musculus
2.8.2.20 30
-
 assay at Homo sapiens
2.8.2.20 30
-
 assay at Rattus norvegicus
2.8.2.20 30
-
 assay at Oryctolagus cuniculus
2.8.2.20 30
-
 assay at Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.20 6.7
-
 liver Rattus norvegicus
2.8.2.20 6.7
-
 sulfation of acidic amino acid polymer EAY Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.20 6.2 7.2 sulfation of acidic amino acid polymer EAY, liver, about half-maximal activity at pH 6.2 and 7.2 Rattus norvegicus