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Literature summary extracted from

  • Singer, S.S.; Bruns, L.
    Enzymatic sulfation of steroids. XI. The extensive purification and some properties of hepatic sulfotransferase III from female rats (1980), Can. J. Biochem., 58, 660-666.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.18 adenosine 3',5'-bisphosphate product inhibition Rattus norvegicus
2.8.2.18 Cd2+ 5 mM, strong inhibition Rattus norvegicus
2.8.2.18 corticosterone cortisol as substrate, strong Rattus norvegicus
2.8.2.18 Cortisol 21-sulfate product inhibition Rattus norvegicus
2.8.2.18 cortisone cortisol as substrate, weak, 0.012 mM Rattus norvegicus
2.8.2.18 dehydroepiandrosterone cortisol as substrate; strong Rattus norvegicus
2.8.2.18 deoxycorticosterone cortisol as substrate, strong Rattus norvegicus
2.8.2.18 dexamethasone cortisol as substrate; weak Rattus norvegicus
2.8.2.18 diethylstilbestrol cortisol as substrate; strong Rattus norvegicus
2.8.2.18 estradiol-17beta 44% inhibition at 0.012 mM; cortisol as substrate Rattus norvegicus
2.8.2.18 progesterone cortisol as substrate; strong Rattus norvegicus
2.8.2.18 testosterone cortisol as substrate, strong Rattus norvegicus
2.8.2.18 tetrahydrocortisol not Rattus norvegicus
2.8.2.18 Zn2+ 5 mM, strong inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.18 0.00648
-
 cortisol pH 6.8 Rattus norvegicus
2.8.2.18 0.00678
-
 3'-phosphoadenylylsulfate pH 6.8 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.18 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.18 Ba2+ activation Rattus norvegicus
2.8.2.18 Ba2+ 5 mM Rattus norvegicus
2.8.2.18 Ba2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Ca2+ activation Rattus norvegicus
2.8.2.18 Ca2+ 5 mM Rattus norvegicus
2.8.2.18 Ca2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Co2+ activation Rattus norvegicus
2.8.2.18 Co2+ 5 mM Rattus norvegicus
2.8.2.18 Co2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Cr2+ activation Rattus norvegicus
2.8.2.18 Cr2+ 5 mM Rattus norvegicus
2.8.2.18 Cr2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Fe3+ not Rattus norvegicus
2.8.2.18 Mg2+ activation Rattus norvegicus
2.8.2.18 Mg2+ 5 mM Rattus norvegicus
2.8.2.18 Mg2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Mn2+ activation Rattus norvegicus
2.8.2.18 Mn2+ 5 mM Rattus norvegicus
2.8.2.18 Mn2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus
2.8.2.18 Ni2+ activation Rattus norvegicus
2.8.2.18 Ni2+ 5 mM Rattus norvegicus
2.8.2.18 Ni2+ in decreasing order of efficiency: Cr2+, Mg2+, Ba2+, Mn2+, Ni2+, Ca2+, Co2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.18 3'-phosphoadenylylsulfate + cortisol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.18 Rattus norvegicus
-
 female Sprague-Dawley CD rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.18 sulfotransferase III Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.18 3'-phosphoadenylyl sulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol 21-sulfate mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.18 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.18 0.016
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.18 -20°C, at least 3 weeks Rattus norvegicus
2.8.2.18 0-4°C, in crude extracts, 3 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.18 3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + cortisol i.e. PAPS, glucocorticoids are preferred substrates Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol 21-sulfate
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + deoxycorticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + estradiol-17beta
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.18 3'-phosphoadenylylsulfate + testosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.18 6
-
 cortisol as substrate Rattus norvegicus