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Literature summary extracted from

  • Schmidt, A.; Christen, U.
    A factor-dependent sulfotransferase specific for 3'-phosphoadenosine-5'-phosphosulfate (PAPS) in the cyanobacterium Synechococus 6301 (1978), Planta, 140, 239-244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.16 2',5'-ADP
-
 Synechococcus sp.
2.8.2.16 3',5'-ADP product inhibition Synechococcus sp.
2.8.2.16 5'-ADP no inhibition Synechococcus sp.
2.8.2.16 5'-AMP no inhibition Synechococcus sp.
2.8.2.16 Mg2+ above 50 mM, activation at 5-10 mM Synechococcus sp.
2.8.2.16 additional information no inhibition by 2'-AMP, 3'-AMP, cAMP; no inhibition by cysteic acid, cysteine or methionine Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.16 0.02
-
 3'-phosphoadenylylsulfate pH 8.0 Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.16 Mg2+ 20% activation at 5-10 mM, severe inhibition above 50 mM Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.16 58000
-
 gel filtration Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.16 3'-phosphoadenylylsulfate + a thiol Synechococcus sp. involved in assimilatory sulfate reduction adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
2.8.2.16 3'-phosphoadenylylsulfate + a thiol Synechococcus sp. 6301 involved in assimilatory sulfate reduction adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.16 Synechococcus sp.
-
 cyanobacterium
-
2.8.2.16 Synechococcus sp. 6301
-
 cyanobacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.16 partial Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.16 3'-phosphoadenylylsulfate + a thiol i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS Synechococcus sp. adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate i.e. 3',5'-ADP ?
2.8.2.16 3'-phosphoadenylylsulfate + a thiol involved in assimilatory sulfate reduction Synechococcus sp. adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
2.8.2.16 3'-phosphoadenylylsulfate + a thiol i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, specific for PAPS Synechococcus sp. 6301 adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate i.e. 3',5'-ADP ?
2.8.2.16 3'-phosphoadenylylsulfate + a thiol involved in assimilatory sulfate reduction Synechococcus sp. 6301 adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
-
 ?
2.8.2.16 3'-phosphoadenylylsulfate + dithioerythritol
-
 Synechococcus sp. ?
-
 ?
2.8.2.16 3'-phosphoadenylylsulfate + dithioerythritol
-
 Synechococcus sp. 6301 ?
-
 ?
2.8.2.16 additional information specificity Synechococcus sp. ?
-
 ?
2.8.2.16 additional information adenosine 5'-phosphosulfate (APS) is no substrate Synechococcus sp. ?
-
 ?
2.8.2.16 additional information specificity Synechococcus sp. 6301 ?
-
 ?
2.8.2.16 additional information adenosine 5'-phosphosulfate (APS) is no substrate Synechococcus sp. 6301 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.16 8
-
-
 Synechococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.16 7 10 about 60% of maximal activity at pH 7.0 and about half-maximal activity at pH 10.0 Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.2.16 thioredoxin requirement Synechococcus sp.