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Literature summary extracted from

  • Park, B.C.; Lee, Y.C.; Strott, C.A.
    Testosterone sulfotransferase: Evidence in the guinea pig that this reaction is carried out by 3alpha-hydroxysteroid sulfotransferase (1999), Steroids, 64, 510-517.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.15 3alphaHST and 3betaHST cDNA cloned and overexpressed in Escherichia coli Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.15 0.000125
-
 17beta-estradiol pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
2.8.2.15 0.00019
-
 allopregnanolone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
2.8.2.15 0.0002
-
 testosterone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
2.8.2.15 0.00025
-
 pregnenolone pH 7.7, 37°C, 3-betaHST isoform Cavia porcellus
2.8.2.15 0.00026
-
 androsterone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
2.8.2.15 0.000788
-
 dehydroepiandrosterone pH 7.7, 37°C, 3-betaHST isoform Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.15 cytosol
-
 Cavia porcellus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone Cavia porcellus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.15 Cavia porcellus
-
 guinea pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.15 TST Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.15 adrenal gland
-
 Cavia porcellus
-
2.8.2.15 liver
-
 Cavia porcellus
-
2.8.2.15 additional information both 3alphaHST and 3betaHST activities are essentially undetectable in the testis Cavia porcellus
-
2.8.2.15 prostate small but detectable amount of 3alphaHST activity Cavia porcellus
-
2.8.2.15 small intestine
-
 Cavia porcellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.15 3'-phosphoadenylylsulfate + 17beta-estradiol 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + ?
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + allopregnanolone
-
 Cavia porcellus adenosine 3',5'-bisphosphate + allopregnanolone sulfate
-
 ?
2.8.2.15 3'-phosphoadenylylsulfate + allopregnenolone 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + allopregnenolone sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Cavia porcellus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + dehydroepiandrosterone 3betaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + pregnenolone 3betaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + pregnenolone sulfate
-
 r
2.8.2.15 3'-phosphoadenylylsulfate + testosterone 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + testosterone sulfate only 3alpha-hydroxysteroid isoform, 3beta-hydroxysteroid isoform exhibits no TST activity r
2.8.2.15 additional information estrone is no substrate Cavia porcellus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.15 3alpha-hydroxysteroid sulfotransferase
-
 Cavia porcellus
2.8.2.15 testosterone sulfotransferase
-
 Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.15 5.5 6 activity with 3alpha-hydroxysteroid Cavia porcellus
2.8.2.15 6.5 7 3betahydroxysteroid activity Cavia porcellus