EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.2.1 | SULT1A1, DNA sequence determination and analysis, expression in Escherichia coli | Homo sapiens |
2.8.2.1 | SULT1A3, wild-type and mutants, expression in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.8.2.1 | E146A | site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 | Homo sapiens |
2.8.2.1 | H143Y | site-directed mutagenesis, reduced activity with dopamine compared to wild-type SULT1A3 | Homo sapiens |
2.8.2.1 | H143Y/E146A | site-directed mutagenesis, reduced activity with dopamine and increased activity with 4-nitrophenol compared to wild-type SULT1A3, switch of specificity to wild-type SULT1A1 | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.1 | 0.002 | - |
3'-phosphoadenylylsulfate | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0022 | - |
dopamine | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.003 | - |
3'-phosphoadenylylsulfate | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0038 | - |
4-nitrophenol | pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0038 | - |
4-nitrophenol | wild-type SULT1A1 | Homo sapiens | |
2.8.2.1 | 0.0043 | - |
3'-phosphoadenylylsulfate | wild-type SULT1A1, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0046 | - |
3'-phosphoadenylylsulfate | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0048 | - |
3'-phosphoadenylylsulfate | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0056 | - |
dopamine | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0061 | - |
4-nitrophenol | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.0094 | - |
4-nitrophenol | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.087 | - |
dopamine | SULT1A3 double mutant H143Y/E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.109 | - |
dopamine | wild-type SULT1A1, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 0.127 | - |
dopamine | SULT1A3 mutant E146A, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 1.38 | - |
4-nitrophenol | wild-type SULT1A3, pH 7.4, 37°C | Homo sapiens | |
2.8.2.1 | 1.47 | - |
4-nitrophenol | SULT1A3 mutant H143Y, pH 7.4, 37°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.1 | Homo sapiens | - |
SULT1A3, is a M-PST enzyme form | - |
2.8.2.1 | Homo sapiens | P50225 | SULT1A1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.1 | recombinant from Escherichia coli | Homo sapiens |
2.8.2.1 | recombinant wild-type SULT1A3 and mutants from Escherichia coli | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.8.2.1 | 3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate | Glu146 determines the substrate specificity | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.2.1 | liver | SULT1A1 | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.1 | 3'-phosphoadenylyl sulfate + a phenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + an aryl sulfate | - |
? | |
2.8.2.1 | 3'-phosphoadenylylsulfate + 4-nitrophenol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate | - |
? | |
2.8.2.1 | 3'-phosphoadenylylsulfate + dopamine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.1 | 3'-phosphoadenylylsulfate + dopamine | SULT1A3 is specific for dopamine and other biogenic amines | Homo sapiens | adenosine 3',5'-bisphosphate + ? | - |
? | |
2.8.2.1 | additional information | substrate specificity | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.2.1 | SULT | - |
Homo sapiens |