Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Whittemore, R.M.; Pearce, L.B.; Roth, J.A.
    Purification and kinetic characterization of a dopamine-sulfating form of phenol sulfotransferase from human brain (1985), Biochemistry, 24, 2477-2482.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 adenosine 3',5'-bisphosphate
-
Homo sapiens
2.8.2.1 ATP M-PST Homo sapiens
2.8.2.1 NaCl 50% inhibition at: 325 mM; M-PST Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.00035
-
3'-phosphoadenylylsulfate M-PST, pH 7.0, 37°C Homo sapiens
2.8.2.1 0.0029
-
dopamine M-PST, pH 7.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 250000
-
isozyme M-PST, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens
-
2 forms: simple phenol sulfating P-PST, monoamine sulfating M-PST
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 monoamine-sulfating form, M-PST Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate isozyme M-PST: ordered bisubstrate reaction mechanism Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 brain
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 additional information
-
-
Homo sapiens
2.8.2.1 0.0077
-
purified M-PST Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
Homo sapiens adenosine 3',5'-bisphosphate + an aryl sulfate
-
?
2.8.2.1 3'-phosphoadenylylsulfate + 4-methoxytyramine
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine
-
Homo sapiens adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + tyramine
-
Homo sapiens adenosine 3',5'-bisphosphate + tyramine O-sulfate
-
?
2.8.2.1 additional information substrate specificity Homo sapiens ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7
-
M-PST, with substrate dopamine Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.1 0.00007
-
adenosine 3',5'-bisphosphate M-PST, pH 7.0, 37°C Homo sapiens
2.8.2.1 0.18
-
ATP M-PST, pH 7.0, 37°C Homo sapiens