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Literature summary extracted from

  • Kato, S.; Mihara, H.; Kurihara, T.; Yoshimura, T.; Esaki, N.
    Gene cloning, purification, and characterization of two cyanobacterial NifS homologs driving iron-sulfur cluster formation (2000), Biosci. Biotechnol. Biochem., 64, 2412-2419.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.7 dithiothreitol increase of enzyme activity at 50 mM Synechocystis sp.
2.8.1.7 pyruvate increase of enzyme activity at 5 mM Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.7
-
Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.7 0.28
-
L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
2.8.1.7 0.43
-
L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.
2.8.1.7 0.59
-
L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
2.8.1.7 17
-
L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.7 Iron [Fe2-S2]ferredoxin Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.7 42000
-
2 * 42000, SDS-PAGE Synechocystis sp.
2.8.1.7 87000
-
isoenzyme SsCsd1, gel filtration Synechocystis sp.
2.8.1.7 88000
-
isoenzyme SsCsd2, gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine Synechocystis sp.
-
L-alanine + [enzyme]-S-sulfanylcysteine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Synechocystis sp.
-
PCC6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.7 isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3 Synechocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.7 4.5
-
isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate Synechocystis sp.
2.8.1.7 4.8
-
isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate Synechocystis sp.
2.8.1.7 5.8
-
isoenzyme SsCsd2, with L-selenocysteine as substrate Synechocystis sp.
2.8.1.7 21
-
isoenzyme SsCsd1, with L-selenocysteine as substrate Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
Synechocystis sp. L-alanine + [enzyme]-S-sulfanylcysteine
-
?
2.8.1.7 L-cysteine + [enzyme]-cysteine L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + [enzyme]-S-sulfanylcysteine
-
?
2.8.1.7 L-cysteine sulfinic acid L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + sulfite
-
?
2.8.1.7 L-cystine
-
Synechocystis sp. ?
-
?
2.8.1.7 L-selenocysteine L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not Synechocystis sp. L-alanine + selenium
-
?
2.8.1.7 L-selenocystine
-
Synechocystis sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
2.8.1.7 dimer 2 * 42000, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.7 37
-
assay at Synechocystis sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.7 3.2
-
L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.
2.8.1.7 3.4
-
L-cysteine sulfinic acid pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
2.8.1.7 4
-
L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd2 Synechocystis sp.
2.8.1.7 15
-
L-selenocysteine pH 7.5, 37°C, isoenzyme SsCsd1 Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.7 7
-
isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates Synechocystis sp.
2.8.1.7 7.7
-
isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate Synechocystis sp.