EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.1.7 | dithiothreitol | increase of enzyme activity at 50 mM | Synechocystis sp. | |
2.8.1.7 | pyruvate | increase of enzyme activity at 5 mM | Synechocystis sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.1.7 | - |
Synechocystis sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.1.7 | 0.28 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
2.8.1.7 | 0.43 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. | |
2.8.1.7 | 0.59 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
2.8.1.7 | 17 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.1.7 | Iron | [Fe2-S2]ferredoxin | Synechocystis sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.1.7 | 42000 | - |
2 * 42000, SDS-PAGE | Synechocystis sp. |
2.8.1.7 | 87000 | - |
isoenzyme SsCsd1, gel filtration | Synechocystis sp. |
2.8.1.7 | 88000 | - |
isoenzyme SsCsd2, gel filtration | Synechocystis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.7 | L-cysteine + [enzyme]-cysteine | Synechocystis sp. | - |
L-alanine + [enzyme]-S-sulfanylcysteine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.1.7 | Synechocystis sp. | - |
PCC6803 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.1.7 | isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3 | Synechocystis sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.1.7 | 4.5 | - |
isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate | Synechocystis sp. |
2.8.1.7 | 4.8 | - |
isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate | Synechocystis sp. |
2.8.1.7 | 5.8 | - |
isoenzyme SsCsd2, with L-selenocysteine as substrate | Synechocystis sp. |
2.8.1.7 | 21 | - |
isoenzyme SsCsd1, with L-selenocysteine as substrate | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.7 | L-cysteine + [enzyme]-cysteine | - |
Synechocystis sp. | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
2.8.1.7 | L-cysteine + [enzyme]-cysteine | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
2.8.1.7 | L-cysteine sulfinic acid | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + sulfite | - |
? | |
2.8.1.7 | L-cystine | - |
Synechocystis sp. | ? | - |
? | |
2.8.1.7 | L-selenocysteine | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + selenium | - |
? | |
2.8.1.7 | L-selenocystine | - |
Synechocystis sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.1.7 | dimer | 2 * 42000, SDS-PAGE | Synechocystis sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.1.7 | 37 | - |
assay at | Synechocystis sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.1.7 | 3.2 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. | |
2.8.1.7 | 3.4 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
2.8.1.7 | 4 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
2.8.1.7 | 15 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.1.7 | 7 | - |
isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates | Synechocystis sp. |
2.8.1.7 | 7.7 | - |
isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate | Synechocystis sp. |