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Literature summary extracted from

  • Picciocchi, A.; Douce, R.; Alban, C.
    Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis (2001), Plant Physiol., 127, 1224-1233.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.6 D-fructose 1,6-bisphosphate enhances biotin formation Arabidopsis thaliana
2.8.1.6 dithiothreitol required Arabidopsis thaliana
2.8.1.6 L-cysteine highly stimulates Arabidopsis thaliana
2.8.1.6 additional information one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants Arabidopsis thaliana
2.8.1.6 S-adenosyl-L-methionine required Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6
-
 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.6 acidomycin structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.6 0.03
-
 dethiobiotin pH 8, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 Fe2+ highly stimulates Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur
-
 Arabidopsis thaliana biotin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.6 0.00055
-
 dethiobiotin pH 8, 37°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.6 NADH required Arabidopsis thaliana
2.8.1.6 NADPH required Arabidopsis thaliana