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Literature summary extracted from

  • Flint, D.H.; Allen, R.M.
    Purification and characterization of biotin synthases (1997), Methods Enzymol., 279, 349-356.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.6 S-adenosyl-L-methionine
-
 Lysinibacillus sphaericus
2.8.1.6 S-adenosyl-L-methionine required Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.6 Iron presence of a [2Fe-2S] cluster Escherichia coli
2.8.1.6 Iron presence of a [2Fe-2S] cluster Lysinibacillus sphaericus
2.8.1.6 Iron the 104000 Da dimeric enzyme form contains a single [2Fe-2S] cluster per dimer Escherichia coli
2.8.1.6 Iron the 82000 Da dimeric enzyme form contains one [2Fe-2S]cluster per monomer Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.6 39000
-
 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE Escherichia coli
2.8.1.6 82000
-
 at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da Escherichia coli
2.8.1.6 104000
-
 at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da Escherichia coli
2.8.1.6 160000
-
 at least 3 forms of the enzyme: 82000 Da, 104000 Da, 160000 Da Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur Escherichia coli catalyzes the last step of the biosynthesis of biotin biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur Lysinibacillus sphaericus catalyzes the last step of the biosynthesis of biotin biotin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-
2.8.1.6 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.6
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.6 dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin radical chemistry is involved in the mechanism of biotin synthase Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur
-
 Escherichia coli biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur
-
 Lysinibacillus sphaericus biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur catalyzes the last step of the biosynthesis of biotin Escherichia coli biotin
-
 ?
2.8.1.6 dethiobiotin + sulfur catalyzes the last step of the biosynthesis of biotin Lysinibacillus sphaericus biotin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.6 dimer
-
 Lysinibacillus sphaericus
2.8.1.6 dimer 2 * 39000, 82000 MW form and 104000 MW form, SDS-PAGE Escherichia coli