Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sugio, T.; Kishimoto, K.; Oda, K.
    Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization (1997), Biosci. Biotechnol. Biochem., 61, 470-474.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.5 EDTA
-
 thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 o-phenanthroline
-
 thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 Sodium cyanide 2.5fold increase at 1 mM thermophilic iron-oxidizing bacterium strain TI-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.5 4-chloromercuribenzene sufonic acid strong inhibition at 0.1 mM thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 Co2+ slight inhibition at 1 mM thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 Ni2+ slight inhibition at 1 mM thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 Zn2+ slight inhibition at 1 mM thermophilic iron-oxidizing bacterium strain TI-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.5 0.125
-
 NADH pH 6, 45°C thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 0.29
-
 thiosulfate pH 6, 45°C thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 5
-
 NADPH pH 6, 45°C thermophilic iron-oxidizing bacterium strain TI-1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.5 cytosol
-
 thermophilic iron-oxidizing bacterium strain TI-1 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.5 58000
-
 homotetramer, 4 * 58000, SDS-PAGE thermophilic iron-oxidizing bacterium strain TI-1
2.8.1.5 243000
-
 gel filtration thermophilic iron-oxidizing bacterium strain TI-1

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.5 thermophilic iron-oxidizing bacterium strain TI-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.5
-
 thermophilic iron-oxidizing bacterium strain TI-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.5 thiosulfate + NAD(P)H
-
 thermophilic iron-oxidizing bacterium strain TI-1 H2S + NAD(P)+ + sulfite
-
 ?
2.8.1.5 thiosulfate + NADH
-
 thermophilic iron-oxidizing bacterium strain TI-1 H2S + NAD+ + sulfite
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.5 tetramer homotetramer, 4 * 58000, SDS-PAGE thermophilic iron-oxidizing bacterium strain TI-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.5 60
-
-
 thermophilic iron-oxidizing bacterium strain TI-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.1.5 100
-
 no enzyme activity after 15 min thermophilic iron-oxidizing bacterium strain TI-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.5 6
-
-
 thermophilic iron-oxidizing bacterium strain TI-1