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Literature summary extracted from

  • Williams, R.A.; Kelly, S.M.; Mottram, J.C.; Coombs, G.H.
    3-Mercaptopyruvate sulfurtransferase of Leishmania contains an unusual C-terminal extension and is involved in thioredoxin and antioxidant metabolism (2003), J. Biol. Chem., 278, 1480-1486.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.2
-
Leishmania mexicana
2.8.1.2 wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 0.2
-
3-Mercaptopyruvate pH 7.3, 37°C Leishmania major
2.8.1.2 0.3
-
reduced thioredoxin pH 7.3, 37°C Leishmania major
2.8.1.2 1.7
-
thiosulfate pH 7.3, 37°C Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.2 cytosol
-
Leishmania major 5829
-
2.8.1.2 cytosol
-
Leishmania mexicana 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.2 40000
-
SDS-PAGE Leishmania major
2.8.1.2 40000
-
SDS-PAGE Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide Leishmania major
-
pyruvate + thiocyanate
-
?
2.8.1.2 3-mercaptopyruvate + cyanide Leishmania mexicana
-
pyruvate + thiocyanate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Leishmania major Q7K9G0
-
-
2.8.1.2 Leishmania mexicana Q8T381
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.2
-
Leishmania mexicana
2.8.1.2 wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus Leishmania major

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.1.2 enzyme regains full activity without the need for assistance in the form of a chaperone or detergent after denaturation is 6 M urea and renaturation using dialysis or dilution Leishmania major

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 amastigote
-
Leishmania major
-
2.8.1.2 promastigote
-
Leishmania major
-
2.8.1.2 promastigote
-
Leishmania mexicana
-

Storage Stability

EC Number Storage Stability Organism
2.8.1.2 -20°C, stable for at least 12 months without loss of activity Leishmania major
2.8.1.2 -20°C, stable for at least 12 months without loss of activity Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide
-
Leishmania major pyruvate + thiocyanate
-
?
2.8.1.2 3-mercaptopyruvate + cyanide
-
Leishmania mexicana pyruvate + thiocyanate
-
?
2.8.1.2 sulfane sulfur + cyanide
-
Leishmania major thiocyanate + ?
-
?
2.8.1.2 sulfane sulfur + cyanide
-
Leishmania mexicana thiocyanate + ?
-
?
2.8.1.2 thiosulfate + thioredoxin
-
Leishmania major ?
-
?
2.8.1.2 thiosulfate + thioredoxin
-
Leishmania mexicana ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.2 6.9 7.6
-
Leishmania major
2.8.1.2 6.9 7.6
-
Leishmania mexicana