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Literature summary extracted from

  • Ray, W.K.; Zeng, G.; Potters, M.B.; Mansuri, A.M.; Larson, T.J.
    Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin (2000), J. Bacteriol., 182, 2277-2284.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.1 5,5'-dithiobis(2-nitrobenzoic acid)
-
Escherichia coli
2.8.1.1 thiosulfate substrate inhibition at low concentrations of cyanide Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 0.034
-
thioredoxin 2°C, pH 8.6 Escherichia coli
2.8.1.1 17
-
CN- 2°C, pH 8.6 Escherichia coli
2.8.1.1 78
-
thiosulfate 2°C, pH 8.6 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 12100
-
alpha2, 2 * 12100, calculated from nucleotide sequence and SDS-PAGE Escherichia coli
2.8.1.1 22500
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Escherichia coli
-
sulfite + thiocyanate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1 GlpE Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 21
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thioredoxin + thiosulfate
-
Escherichia coli ?
-
?
2.8.1.1 thiosulfate + cyanide
-
Escherichia coli sulfite + thiocyanate
-
?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 dimer alpha2, 2 * 12100, calculated from nucleotide sequence and SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.1 230
-
thiosulfate 2°C, pH 8.6 Escherichia coli