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Literature summary extracted from

  • Pagani, S.; Forlani, F.; Carpen, A.; Bordo, D.; Colnaghi, R.
    Mutagenic analysis of Thr-232 in rhodanese from Azotobacter vinelandii highlighted the differences of this prokaryotic enzyme from the known sulfurtransferases (2000), FEBS Lett., 472, 307-311.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.1 overexpression in Escherichia coli M15 Azotobacter vinelandii

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.1 K249A complete inactivation of rhodanese with thiosulfate Bos taurus
2.8.1.1 T232A increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor Azotobacter vinelandii
2.8.1.1 T232K increase in thiosulfate-cyanide sulfurtransferase activity and no activity with 3-mercaptopyruvate as sulfur donor Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide Azotobacter vinelandii
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Azotobacter vinelandii
-
-
-
2.8.1.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1
-
 Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 225
-
 recombinant wild-type enzyme Azotobacter vinelandii
2.8.1.1 440
-
 T232A Azotobacter vinelandii
2.8.1.1 550
-
 T232K Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Azotobacter vinelandii sulfite + thiocyanate
-
 ?