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Literature summary extracted from

  • Hutchins, A.M.; Holden, J.F.; Adams, M.W.
    Phosphoenolpyruvate synthetase from the hyperthermophilic archaeon Pyrococcus furiosus (2001), J. Bacteriol., 183, 709-715.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.2 additional information not affected by Na+ Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.2 additional information
-
 additional information kinetics Pyrococcus furiosus
2.7.9.2 0.11
-
 pyruvate pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 0.39
-
 ATP pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 0.4
-
 phosphoenolpyruvate pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 1
-
 AMP pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 38.4
-
 phosphate pH 8.4, 80°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.2 cytoplasm 5% of total cytoplasmic protein Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.2 Ca2+ enzyme contains 1 Ca atom per subunit Pyrococcus furiosus
2.7.9.2 Co2+ requires divalent cations in the forward reaction, 93% of the activity with Mg2+ Pyrococcus furiosus
2.7.9.2 Iron 0.1-0.4 g-atoms per subunit, no functional part of the enzyme Pyrococcus furiosus
2.7.9.2 K+ 50% increased activity at 100 mM Pyrococcus furiosus
2.7.9.2 Mg2+ slightly inhibitory above 10 mM Pyrococcus furiosus
2.7.9.2 Mg2+ requires divalent cations in the forward reaction, Mg2+ is most effective Pyrococcus furiosus
2.7.9.2 Mn2+ requires divalent cations in the forward reaction, 95% of the activity with Mg2+ Pyrococcus furiosus
2.7.9.2 additional information Ca2+ and Sr2+ cannot substitute for Mg2+ in the catalytic reaction Pyrococcus furiosus
2.7.9.2 additional information not affected by Na+ Pyrococcus furiosus
2.7.9.2 NH4+ 48% increased activity at 100 mM Pyrococcus furiosus
2.7.9.2 Ni2+ requires divalent cations in the forward reaction, 15% of the activity with Mg2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.2 92000
-
 8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa Pyrococcus furiosus
2.7.9.2 690000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.2 ATP + pyruvate + H2O Pyrococcus furiosus involved in gluconeogenesis AMP + phosphoenolpyruvate + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.2 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.2 19.4fold Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.2 14.9
-
 purified enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.2 ATP + 2-ketobutyrate + H2O 8% of the activity with pyruvate Pyrococcus furiosus ?
-
 ?
2.7.9.2 ATP + 2-oxoglutarate + H2O 2% of the activity with pyruvate Pyrococcus furiosus ?
-
 ?
2.7.9.2 ATP + pyruvate + H2O absolutely specific for ATP, highly specific for pyruvate Pyrococcus furiosus AMP + phosphoenolpyruvate + phosphate
-
 r
2.7.9.2 ATP + pyruvate + H2O phosphorylated enzyme as an intermediate Pyrococcus furiosus AMP + phosphoenolpyruvate + phosphate
-
 r
2.7.9.2 ATP + pyruvate + H2O equilibrium lies far to the side of phosphoenolpyruvate formation Pyrococcus furiosus AMP + phosphoenolpyruvate + phosphate
-
 r
2.7.9.2 ATP + pyruvate + H2O involved in gluconeogenesis Pyrococcus furiosus AMP + phosphoenolpyruvate + phosphate
-
 r
2.7.9.2 additional information no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.2 octamer 8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.7.9.2 PpsA
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.2 90
-
 at pH 8.4 Pyrococcus furiosus
2.7.9.2 90
-
 phosphoenolpyruvate formation Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.9.2 30 90 optimum at 90°C, 0.2% of the optimal activity at 30°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.9.2 90
-
 t1/2: 9 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.9.2 8.7
-
 AMP pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 11.9
-
 phosphate pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 12.6
-
 phosphoenolpyruvate pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 1330
-
 ATP pH 8.4, 80°C Pyrococcus furiosus
2.7.9.2 1570
-
 pyruvate pH 8.4, 80°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.2 7.5
-
 at 80°C Pyrococcus furiosus
2.7.9.2 7.5
-
 pyruvate formation Pyrococcus furiosus
2.7.9.2 9
-
 at 80°C Pyrococcus furiosus
2.7.9.2 9
-
 phosphoenolpyruvate formation Pyrococcus furiosus