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Literature summary extracted from

  • Bringaud, F.; Baltz, D.; Baltz, T.
    Functional and molecular characterization of a glycosomal PPi-dependent enzyme in trypanosomatids: pyruvate, phosphate dikinase (1998), PROC. NATL. ACAD. SCI. USA, 95, 7963-7968.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 expressed in Escherichia coli Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.0075
-
 AMP
-
 Trypanosoma brucei
2.7.9.1 0.04
-
 phosphoenolpyruvate
-
 Trypanosoma brucei
2.7.9.1 0.05
-
 diphosphate
-
 Trypanosoma brucei
2.7.9.1 0.3
-
 pyruvate
-
 Trypanosoma brucei
2.7.9.1 0.5
-
 phosphate
-
 Trypanosoma brucei
2.7.9.1 0.6
-
 ATP
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.1 glycosome
-
 Trypanosoma brucei 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 K+ not required for activity Trypanosoma brucei
2.7.9.1 Mg2+ required for activity Trypanosoma brucei
2.7.9.1 NH4+ not required for activity Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 100000
-
 antibody recognition Trypanosoma cruzi
2.7.9.1 100000
-
 antibody recognition Leishmania sp.
2.7.9.1 100000
-
 antibody recognition Trypanosoma congolense
2.7.9.1 100000
-
 antibody recognition Trypanosoma brucei
2.7.9.1 100000
-
 antibody recognition Trypanosoma vivax
2.7.9.1 100000
-
 antibody recognition Crithidia sp.
2.7.9.1 100000
-
 antibody recognition Phytomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Crithidia sp.
-
-
-
2.7.9.1 Leishmania sp.
-
-
-
2.7.9.1 Phytomonas sp.
-
-
-
2.7.9.1 Trypanosoma brucei O76283
-
-
2.7.9.1 Trypanosoma congolense
-
-
-
2.7.9.1 Trypanosoma cruzi
-
-
-
2.7.9.1 Trypanosoma vivax
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 2.5
-
 catalysis of both the pyruvate and the phosphoenolpyruvate formation Trypanosoma brucei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.9.1 Trypanosoma brucei calculated
-
 8.25