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Literature summary extracted from

  • Flugel, R.S.; Hwangbo, Y.; Lambalot, R.H.; Cronan, J.E., Jr.; Walsh, C.T.
    Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli (2000), J. Biol. Chem., 275, 959-968.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 gene acpS cloned and expressed in Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 apo-acyl-carrier protein mutant ACP in which the target serine 36 has been mutated to a threonine residue is an inhibitor as well as an inactive substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0015
-
 apo-acyl-carrier protein pH 8.8, 37°C Escherichia coli
2.7.8.7 0.05
-
 CoA pH 8.8, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Escherichia coli
-
 K-12 derivatives
-
2.7.8.7 no activity in Escherichia coli
-
 denoted acpS1 gene
-
2.7.8.7 no activity in Escherichia coli MP4
-
 denoted acpS1 gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 additional information mutant ACP in which the target serine 36 has been mutated to a threonine residue is an inactive substrate for phosphopantetheinylation Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 AcpS
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 1.03
-
 apo-acyl-carrier protein pH 8.8, 37°C, 250 mM NaCl Escherichia coli
2.7.8.7 1.08
-
 apo-acyl-carrier protein pH 8.8, 37°C Escherichia coli