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Literature summary extracted from

  • Elovson, J.; Vagelos, P.R.
    Acyl carrier protein. X. Acyl carrier protein synthetase (1968), J. Biol. Chem., 243, 3603-3611.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0004
-
 apo-acyl-carrier protein pH 8.0, 33°C Escherichia coli
2.7.8.7 0.002
-
 apo-peptide(1-->74) pH 8.0, 33°C Escherichia coli
2.7.8.7 0.15
-
 CoA pH 8.0, 33°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ requires Mg2+ or Mn2+ for activity, Km for Mg2+ 3.0 mM Escherichia coli
2.7.8.7 Mn2+ requires Mn2+ or Mg2+ for activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli B / ATCC 11303 ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Escherichia coli
-
-
-
2.7.8.7 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.7 additional information
-
 specific activity 88.0 units/mg protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] transfers 4'-phosphopantetheine from reduced coenzyme A to acyl carrier proteon apoprotein Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] transfers 4'-phosphopantetheine from reduced coenzyme A to acyl carrier proteon apoprotein Escherichia coli B / ATCC 11303 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids Escherichia coli B / ATCC 11303 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 additional information specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive Escherichia coli ?
-
 ?
2.7.8.7 additional information specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive Escherichia coli B / ATCC 11303 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 7.5 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.7 6 11 about 20% of activity maximum at pH 6.5, about 30% of activity maximum at pH 11.0 Escherichia coli