BRENDA - Enzyme Database

The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier

Fiedler, F.; Glaser, L.; J. Biol. Chem. 249, 2684-2689 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.14
cardiolipin
stimulates 6fold at 0.36 mM
Staphylococcus aureus
2.7.8.14
lipoteichoic acid
required as carrier
Staphylococcus aureus
2.7.8.14
phosphatidylcholine
stimulates 2.7fold 0.36 mM
Staphylococcus aureus
2.7.8.14
phosphatidylethanolamine
stimulates 5fold 0.36 mM
Staphylococcus aureus
2.7.8.14
phosphatidylglycerol
stimulates 4fold 0.36 mM
Staphylococcus aureus
2.7.8.14
Phospholipid
required
Staphylococcus aureus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.14
4-hydroxymercuribenzoate
inhibition at 0.4 mM, can be reversed by 2-mercaptoethanol
Staphylococcus aureus
2.7.8.14
CDPglycerol
weak
Staphylococcus aureus
2.7.8.14
CMP
-
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.14
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus
-
CMP + (ribitol phosphate)n+1
-
-
?
2.7.8.14
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus H
-
CMP + (ribitol phosphate)n+1
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.14
Staphylococcus aureus
-
H
-
2.7.8.14
Staphylococcus aureus H
-
H
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.14
400fold
Staphylococcus aureus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.14
0.0035
-
-
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.14
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus
CMP + (ribitol phosphate)n+1
-
-
-
?
2.7.8.14
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus H
CMP + (ribitol phosphate)n+1
-
-
-
?
2.7.8.14
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
2.7.8.14
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus H
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.14
25
-
24 h, very stable
Staphylococcus aureus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.14
cardiolipin
stimulates 6fold at 0.36 mM
Staphylococcus aureus
2.7.8.14
lipoteichoic acid
required as carrier
Staphylococcus aureus
2.7.8.14
phosphatidylcholine
stimulates 2.7fold 0.36 mM
Staphylococcus aureus
2.7.8.14
phosphatidylethanolamine
stimulates 5fold 0.36 mM
Staphylococcus aureus
2.7.8.14
phosphatidylglycerol
stimulates 4fold 0.36 mM
Staphylococcus aureus
2.7.8.14
Phospholipid
required
Staphylococcus aureus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.14
4-hydroxymercuribenzoate
inhibition at 0.4 mM, can be reversed by 2-mercaptoethanol
Staphylococcus aureus
2.7.8.14
CDPglycerol
weak
Staphylococcus aureus
2.7.8.14
CMP
-
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.14
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus
-
CMP + (ribitol phosphate)n+1
-
-
?
2.7.8.14
CDPribitol + (ribitol phosphate)n
Staphylococcus aureus H
-
CMP + (ribitol phosphate)n+1
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.14
400fold
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.14
0.0035
-
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.14
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus
CMP + (ribitol phosphate)n+1
-
-
-
?
2.7.8.14
CDPribitol + (ribitol phosphate)n
-
645346
Staphylococcus aureus H
CMP + (ribitol phosphate)n+1
-
-
-
?
2.7.8.14
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
2.7.8.14
CDPribitol + lipoteichoic acid
-
645346
Staphylococcus aureus H
lipoteichoic acid-(P-ribitol)n + CMP
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.14
25
-
24 h, very stable
Staphylococcus aureus