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Literature summary extracted from

  • Tanaka, H.; Oiwa, R.; Matsukura, S.; Inokoshi, J.; Omura, S.
    Studies on bacterial cell wall inhibitors. X. Properties of phosph-N-acetylmuramoyl-pentapeptide-transferase in peptidoglycan synthesis of Bacillus megaterium and its inhibition by amphomycin (1982), J. Antibiot., 35, 1216-1221.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.13 Amphomycin
-
 Priestia megaterium
2.7.8.13 Cs+
-
 Priestia megaterium
2.7.8.13 NH4+
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.13 0.093
-
 UDP-MurNAc-pentapeptide pH 8.5, 25°C, enzyme is not purified Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.13 Mg2+ required for maximal activation, optimum concentration 25 mM Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor Priestia megaterium
-
 UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor Priestia megaterium KM / ATCC 13632
-
 UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.13 Priestia megaterium
-
-
-
2.7.8.13 Priestia megaterium KM / ATCC 13632
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.13 UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
-
 Priestia megaterium UMP + [2H]UDP-NAc-muramoyl-pentapeptide
-
 r
2.7.8.13 UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
-
 Priestia megaterium KM / ATCC 13632 UMP + [2H]UDP-NAc-muramoyl-pentapeptide
-
 r
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor
-
 Priestia megaterium UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor
-
 Priestia megaterium KM / ATCC 13632 UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.13 8.5
-
-
 Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.13 5.5 9 activity is significantly weaker at values below pH7.5 or over pH 9.0, about 40% of activity maximum at pH 6.5, about 80% of activity maximum at pH 9.0 Priestia megaterium