BRENDA - Enzyme Database

Determination of the rate-limiting segment of aminoglycoside nucleotidyltransferase 2''-I by pH- and viscosity-dependent kinetics

Gates, C.A.; Northrop, D.B.; Biochemistry 27, 3834-3842 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.46
gentamycin C1
-
Escherichia coli
2.7.7.46
netilmicin
substrate inhibition
Escherichia coli
2.7.7.46
sisomicin
uncompetitive substrate inhibition
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.7.46
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.7.46
ATP + netilmicin C
-
645290
Escherichia coli
diphosphate + 2''-adenylylnetilmicin C
-
-
-
r
2.7.7.46
ATP + sisomicin
-
645290
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.7.46
aminoglycoside nucleotidyltransferase 2''-I
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.46
6
9.5
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.46
0.154
-
sisomicin
-
Escherichia coli
2.7.7.46
1.84
-
gentamycin C1
-
Escherichia coli
2.7.7.46
5.12
-
netilmicin
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.46
gentamycin C1
-
Escherichia coli
2.7.7.46
netilmicin
substrate inhibition
Escherichia coli
2.7.7.46
sisomicin
uncompetitive substrate inhibition
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.7.46
0.154
-
sisomicin
-
Escherichia coli
2.7.7.46
1.84
-
gentamycin C1
-
Escherichia coli
2.7.7.46
5.12
-
netilmicin
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.7.46
ATP + netilmicin C
-
645290
Escherichia coli
diphosphate + 2''-adenylylnetilmicin C
-
-
-
r
2.7.7.46
ATP + sisomicin
-
645290
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.46
6
9.5
-
Escherichia coli