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Literature summary extracted from

  • Van Pelt, J.E.; Northrop, D.B.
    Purification and properties of gentamicin nucleotidyltransferase from Escherichia coli: nucleotide specificity, pH optimum, and the separation of two electrophoretic variants (1984), Arch. Biochem. Biophys., 230, 250-263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.46 MgATP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgCTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgdATP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgdCTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgdGTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgdTTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
2.7.7.46 MgGTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.46 0.00276
-
 tobramycin pH 9.0, 25°C Escherichia coli
2.7.7.46 0.03
-
 dATP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.03
-
 dGTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.03
-
 dATP dATP in form of MgdATP2- Escherichia coli
2.7.7.46 0.03
-
 dGTP dGTP in form of MgdGTP2- Escherichia coli
2.7.7.46 0.09
-
 dTTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.09
-
 dCTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.09
-
 dTTP dTTP in form of MgdTTP2- Escherichia coli
2.7.7.46 0.09
-
 dCTP dCTP in form of MgdCTP2- Escherichia coli
2.7.7.46 0.404
-
 ATP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.404
-
 ATP ATP in form of MgATP2- Escherichia coli
2.7.7.46 2.008
-
 CTP pH 9.0, 25°C Escherichia coli
2.7.7.46 2.008
-
 CTP CTP in form of MgCTP2- Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.46 Mg2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.46 31500
-
 isoform II, SDS-PAGE Escherichia coli
2.7.7.46 32500
-
 isoform I, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.46 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.46 2 related isoforms Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.46 0.134
-
 isoform I Escherichia coli
2.7.7.46 0.274
-
 isoform II Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.46 ATP + tobramycin
-
 Escherichia coli diphosphate + 2''-adenylyltobramycin
-
 r
2.7.7.46 CTP + tobramycin
-
 Escherichia coli diphosphate + 2''-cytosylyltobramycin
-
 r
2.7.7.46 dATP + tobramycin
-
 Escherichia coli diphosphate + 2''-deoxyadenylyltobramycin
-
 r
2.7.7.46 dCTP + tobramycin
-
 Escherichia coli diphosphate + 2''-deoxycytosylyltobramycin
-
 r
2.7.7.46 dGTP + tobramycin
-
 Escherichia coli diphosphate + 2''-deoxyguanosylyltobramycin
-
 r
2.7.7.46 dTTP + tobramycin
-
 Escherichia coli diphosphate + 2''-deoxythymidylyltobramycin
-
 r
2.7.7.46 GTP + tobramycin
-
 Escherichia coli diphosphate + 2''-guanosylyltobramycin
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.46 monomer 1 * 32500, isoform I, SDS-PAGE Escherichia coli
2.7.7.46 monomer 1 * 31500, isoform II, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.7.46 AAD 2''
-
 Escherichia coli
2.7.7.46 ANT2''
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.46 9.5
-
 isoform I Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.46 6.5 10
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.46 0.01
-
 MgGTP2- pH 9.0, 25°C Escherichia coli
2.7.7.46 0.011
-
 MgdATP2- pH 9.0, 25°C Escherichia coli
2.7.7.46 0.027
-
 MgdGTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.047
-
 MgdCTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.145
-
 MgdTTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.404
-
 MgCTP pH 9.0, 25°C Escherichia coli
2.7.7.46 0.864
-
 MgATP2- pH 9.0, 25°C Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.46 Escherichia coli 2 related isoforms, isoelectric focusing
-
 5.7
2.7.7.46 Escherichia coli 2 related isoforms, isoelectric focusing
-
 6