EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | 2-mercaptoethanol | 5 mM, activation to 138% of original activity | Rattus norvegicus | |
2.7.7.43 | dithiothreitol | 5 mM, activates to 193% of the original activity | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.7.7.43 | purified enzyme is sensitive to repeated freezing | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | (NH4)2SO4 | 1.0 M, 83% inhibition, reversible by desalting | Rattus norvegicus | |
2.7.7.43 | AMP | 10 mM in presence of 3 mM CTP, 43% inhibition | Rattus norvegicus | |
2.7.7.43 | CDP | 10 mM in presence of 3 mM CTP, 73% inhibition | Rattus norvegicus | |
2.7.7.43 | CMP | 10 mM in presence of 3 mM CTP, 89% inhibition | Rattus norvegicus | |
2.7.7.43 | CMP-N-acylneuraminate | 5 mM, 60% inhibition | Rattus norvegicus | |
2.7.7.43 | CTP | at high concentrations | Rattus norvegicus | |
2.7.7.43 | Cu2+ | 10 mM, 98% inhibition | Rattus norvegicus | |
2.7.7.43 | Fe3+ | 10 mM, 50% inhibition | Rattus norvegicus | |
2.7.7.43 | GTP | 10 mM in presence of 3 mM CTP, 63% inhibition | Rattus norvegicus | |
2.7.7.43 | Hg2+ | 10 mM, 62% inhibition | Rattus norvegicus | |
2.7.7.43 | TTP | 10 mM in presence of 3 mM CTP, 71% inhibition | Rattus norvegicus | |
2.7.7.43 | UTP | 10 mM in presence of 3 mM CTP, 75% inhibition | Rattus norvegicus | |
2.7.7.43 | Zn2+ | 10 mM, 98% of maximal activity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 1.3 | - |
N-acetylneuraminate | pH 8.0, 37°C | Rattus norvegicus | |
2.7.7.43 | 1.5 | - |
CTP | pH 8.0, 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.43 | nucleus | - |
Rattus norvegicus | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | Ca2+ | 33% of the activation with Mg2+ | Rattus norvegicus | |
2.7.7.43 | Co2+ | 80% of the activation with Mg2+ | Rattus norvegicus | |
2.7.7.43 | Mg2+ | maximal activity at 10 mM | Rattus norvegicus | |
2.7.7.43 | Mn2+ | 70% of the activity with Mg2+ | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
2.7.7.43 | 116000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.43 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.43 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.43 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 2 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.43 | -20°C, 2 weeks, more than 50% loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | CTP + N-acylneuraminate | - |
Rattus norvegicus | diphosphate + CMP-N-acylneuraminate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.43 | dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 45 | - |
- |
Rattus norvegicus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 90% of maximal activity | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 35 | - |
60 min, completely stable | Rattus norvegicus |
2.7.7.43 | 37 | - |
60 min, about 10% loss of activity | Rattus norvegicus |
2.7.7.43 | 40 | - |
60 min, 20% loss of activity | Rattus norvegicus |
2.7.7.43 | 45 | - |
60 min, 65% loss of activity, less than 10% loss of activity in presence of 20% w/v glycerol | Rattus norvegicus |
2.7.7.43 | 50 | - |
10 min, 85% loss of activity | Rattus norvegicus |
2.7.7.43 | 55 | - |
20 min, complete loss of activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 8 | - |
at an ionic strength ranging between 50-250 mM, in Tris-HCl buffer, glycine-NaOH as well as in phosphate buffer | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 7 | 10 | pH 7.0: about 90% of maximal activity, pH 10.0: about 70% of maximal activity | Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.7.43 | Rattus norvegicus | chromatofocusing | - |
6.5 |