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Literature summary extracted from

  • Ray, P.H.; Benedict, C.D.
    CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) (1982), Methods Enzymol., 83, 535-540.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.2
-
 CTP pH 9.5, 30°C Escherichia coli
2.7.7.38 0.39
-
 3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Co2+ much less effective than Mg2+ Escherichia coli
2.7.7.38 Mg2+ requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective Escherichia coli
2.7.7.38 Mn2+ much less effective than Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.38 35000
-
 molecular sieving Escherichia coli
2.7.7.38 36000
-
 1 * 36000, SDS-PAGE Escherichia coli
2.7.7.38 44000
-
 sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Escherichia coli
-
-
-
2.7.7.38 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.38 6.9
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.38 -90°C, purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, no loss detected for up to 4 months Escherichia coli
2.7.7.38 4°C, partially purified enzyme, no loss of activity for up to 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli B / ATCC 11303 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 Escherichia coli diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 Escherichia coli B / ATCC 11303 diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 additional information dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate Escherichia coli ?
-
 ?
2.7.7.38 additional information dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303 ?
-
 ?
2.7.7.38 UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, approximately 50% the rate with CTP Escherichia coli diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, approximately 50% the rate with CTP Escherichia coli B / ATCC 11303 diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.38 monomer 1 * 36000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 9.5
-
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.38 Escherichia coli isoelectric focusing
-
 4