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Literature summary extracted from

  • Ray, P.H.; Benedict, C.D.; Grasmuk, H.
    Purification and characterization of cytidine 5-triphosphate:cytidine 5-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase (1981), J. Bacteriol., 145, 1273-1280.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.38 diphosphate weak inhibition Escherichia coli
2.7.7.38 Hg2+ strong inhibition Escherichia coli
2.7.7.38 additional information no inhibition by CDP, CMP, 3-deoxy-manno-octulosonate 8-phosphate, N-acetylneuraminate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.2
-
 CTP
-
 Escherichia coli
2.7.7.38 0.29
-
 3-deoxy-D-manno-octulosonate pH 9.5, 30°C Escherichia coli
2.7.7.38 0.34
-
 dCTP pH 8.0, 37°C Escherichia coli
2.7.7.38 0.88
-
 UTP pH 8.0, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.38 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Ba2+ activation, 20% as effective as Mg2+ Escherichia coli
2.7.7.38 Ca2+ activation, 14% as effective as Mg2+ Escherichia coli
2.7.7.38 Cd2+ activation, 42% as effective as Mg2+ Escherichia coli
2.7.7.38 Co2+ activation, 11% as effective as Mg2+ Escherichia coli
2.7.7.38 Mg2+ requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective Escherichia coli
2.7.7.38 Mn2+ activation, 38% as effective as Mg2+ Escherichia coli
2.7.7.38 additional information monovalent cations Na+, Li+ andK+ are ineffective in stimulating the enzyme, as are divalent cations Hg2+, Ni2+, and Fe2+ Escherichia coli
2.7.7.38 Zn2+ activation, 32% as effective as Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.38 35000 45000 sucrose density gradient centrifugation Escherichia coli
2.7.7.38 35000 40000 SDS-PAGE Escherichia coli
2.7.7.38 36000
-
 1 * 36000, SDS-PAGE Escherichia coli
2.7.7.38 40000 46000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli B / ATCC 11303
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Escherichia coli
-
-
-
2.7.7.38 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.38 6.9
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.38 -90°C, partially purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, stable for up to 3 months Escherichia coli
2.7.7.38 4°C, partially purified protein, 0.05 M potassium phosphate buffer, 0.5 mM dithiothreitol, stable for up to 3 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli B / ATCC 11303 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 and 9.5 Escherichia coli diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 dCTP + 3-deoxy-D-manno-octulosonate reaction at pH 8.0 and 9.5 Escherichia coli B / ATCC 11303 diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 additional information no other sugar can replace 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate and N-acetylneuraminic acid will not serve as substrates, neither dUTP nor any other nucleotide triphosphates, diphosphates or monophosphates like ATP, dATP, GTP, dGTP, ITP, dITP, TTP, CDP, UDP or CMP can substitute for CTP Escherichia coli ?
-
 ?
2.7.7.38 additional information no other sugar can replace 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate and N-acetylneuraminic acid will not serve as substrates, neither dUTP nor any other nucleotide triphosphates, diphosphates or monophosphates like ATP, dATP, GTP, dGTP, ITP, dITP, TTP, CDP, UDP or CMP can substitute for CTP Escherichia coli B / ATCC 11303 ?
-
 ?
2.7.7.38 UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, to a lesser extent at pH 8.0 Escherichia coli diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r
2.7.7.38 UTP + 3-deoxy-D-manno-octulosonate reaction at pH 9.5, to a lesser extent at pH 8.0 Escherichia coli B / ATCC 11303 diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.38 monomer 1 * 36000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 9.3
-
 0.2 M glycine NaOH buffer Escherichia coli
2.7.7.38 9.6
-
 0.2 M Tris-acetate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.38 7.5 10.2 about half-maximal activity at pH 7.5 and about 80% of maximal activity at pH 10.2 in Tris-acetate buffer Escherichia coli
2.7.7.38 7.5 10.5 about half-maximal activity at pH 7.5 and about 60% of maximal activity at pH 10.5 in glycine-NaOH buffer Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.38 7.4 8 stable during purification in Tris-acetate or Tris-chloride buffer, more stable in phosphate buffer than in Tris-acetate or Tris-chloride buffer Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.38 Escherichia coli isoelectric focusing 4.4 4.15