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Literature summary extracted from

  • Ishihara, H.; Heath, E.C.
    The metabolism of L-fucose. IV. The biosynthesis of guanosine diphosphate L-fucose in porcine liver (1968), J. Biol. Chem., 243, 1110-1115.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.30 0.12
-
 GDP-L-fucose
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.30 Co2+ can partially replace Mg2+ in activation Sus scrofa
2.7.7.30 Mg2+ divalent cation required, Mg2+ most effective Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.30 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.30 protamine sulfate, ammonium sulfate, calcium phosphate gel, Sephadex G-100, DEAE-cellulose, alumina C-gamma Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.30 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.30 0.0036
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.7.30 -16°C, 10 mM dithioerythritol, 24 hours, 30-35% loss of activity Sus scrofa
2.7.7.30 -16°C, 24 hours, 80% loss of activity Sus scrofa
2.7.7.30 0°C, 24 hours, 85% loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.30 diphosphate + GDP-L-fucose
-
 Sus scrofa GTP + L-fucose 1-phosphate
-
 r
2.7.7.30 GTP + alpha-D-mannose 1-phosphate 17.5% of activity with GDP-L-fucose Sus scrofa diphosphate + GDP-alpha-D-mannopyranose
-
 ?
2.7.7.30 GTP + L-fucose 1-phosphate specific for GTP Sus scrofa diphosphate + GDP-L-fucose
-
 r
2.7.7.30 UTP + L-fucose 1-phosphate 18.7% of activity with GTP Sus scrofa diphosphate + UDP-L-fucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.30 7.8
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.30 6 9.5 approx. 85% of maximal activity at pH 6.0, approx. 70% of maximal activity at pH 9.5 Sus scrofa