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Literature summary extracted from

  • Eyzaguirre, J.; Bazaes, S.
    Phosphomevalonate kinase from pig liver (1985), Methods Enzymol., 110, 78-85.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 0.056
-
 ATP pH 7.5, 30°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Co2+ can partially replace Mg2+ in activation Sus scrofa
2.7.4.2 Mg2+ divalent cation required, Mg2+ most efficient Sus scrofa
2.7.4.2 Mn2+ can partially replace Mg2+ in activation Sus scrofa
2.7.4.2 Zn2+ can partially replace Mg2+ in activation Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.2 21000
-
 1 * 21000, SDS-PAGE Sus scrofa
2.7.4.2 22000
-
-
 Sus scrofa
2.7.4.2 22500
-
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Sus scrofa
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.4.2 very unstable in absence of thiol compounds Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.2 ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate ordered mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.2 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.2 69.1
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.4.2 -20°C, 10 mM Tris-HCl, pH 7.5, 1 mM dithiothreitol, 50% glycerol, 15 months, 20% loss of acctivity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + 5-phosphomevalonate (3R)-phosphomevalonate Sus scrofa ADP + 5-diphosphomevalonate (3R)-diphosphomevalonate r
2.7.4.2 ATP + 5-phosphomevalonate absolute specificity for ATP Sus scrofa ADP + 5-diphosphomevalonate (3R)-diphosphomevalonate r
2.7.4.2 ATP + 5-phosphomevalonate the equilibrium constant is near unity at pH 7.5 and 30°C Sus scrofa ADP + 5-diphosphomevalonate (3R)-diphosphomevalonate r

Subunits

EC Number Subunits Comment Organism
2.7.4.2 monomer 1 * 21000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.2 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 7.5 9.5
-
 Sus scrofa