BRENDA - Enzyme Database

Structures of Escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity

Briozzo, P.; Golinelli-Pimpaneau, B.; Gilles, A.M.; Gaucher, J.F.; Burlacu-Miron, S.; Sakamoto, H.; Janin, J.; Barzu, O.; Structure 6, 1517-1527 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.25
hanging-drop method in 50 mM Tris-HCl buffer, pH 7.4, 20C, with ammonium sulfate as a precipitant
Escherichia coli
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.4.25
enzyme in complex with CDP
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.25
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
-
645163
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
105% of the activity with CMP
645163
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
0.8% of the activity with CMP
645163
Escherichia coli
ADP + UDP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
hanging-drop method in 50 mM Tris-HCl buffer, pH 7.4, 20C, with ammonium sulfate as a precipitant
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.4.25
enzyme in complex with CDP
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
-
645163
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
105% of the activity with CMP
645163
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
0.8% of the activity with CMP
645163
Escherichia coli
ADP + UDP
-
-
-
?