BRENDA - Enzyme Database

Reaction of human UMP-CMP kinase with natural and analog substrates

Pasti, C.; Gallois-Montbrun, S.; Munier-Lehmann, H.; Veron, M.; Gilles, A.M.; Deville-Bonne, D.; Eur. J. Biochem. 270, 1784-1790 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
expression of His-tagged UMP-CMP kinase and UMP-CMP kinase fusion protein with glutathione S-transferase
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
substrate inhibition above 0.2 mM
Homo sapiens
2.7.4.14
UMP
substrate inhibition above 0.2 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.022
-
CMP
pH 7.4, 37C
Homo sapiens
2.7.4.14
0.044
-
UMP
pH 7.4, 37C
Homo sapiens
2.7.4.14
0.045
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.07
-
CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.14
-
UMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.15
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.2
-
CMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
0.2
-
UMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
0.26
-
ara-CMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.3
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.34
-
ara-CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.9
-
dCMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
1.1
-
dCMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
2
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
2
-
cCMP
pH 7.4, 37C
Dictyostelium sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + dCDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + UDP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Dictyostelium sp.
-
-
-
2.7.4.14
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + ara-CMP
-
645162
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + ara-CMP
-
645162
Dictyostelium sp.
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
-
645162
Homo sapiens
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate
-
-
-
?
2.7.4.14
ATP + CMP
-
645162
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
-
645162
Dictyostelium sp.
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645162
Dictyostelium sp.
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
2.7.4.14
ATP + UMP
-
645162
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645162
Dictyostelium sp.
ADP + UDP
-
-
-
-
2.7.4.14
ATP + UMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site
645162
Homo sapiens
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.14
10
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
14
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
22
-
dCMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
30
-
dCMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
36
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
45
-
ara-CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
72
-
CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
73
-
dCMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
92
-
UMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
130
-
CMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
130
-
UMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
150
-
ara-CMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
217
-
UMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
410
-
CMP
pH 7.4, 37C
Dictyostelium sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.4.14
0.12
-
CMP
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
0.5
-
CMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.5
-
UMP
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
1.5
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
expression of His-tagged UMP-CMP kinase and UMP-CMP kinase fusion protein with glutathione S-transferase
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
substrate inhibition above 0.2 mM
Homo sapiens
2.7.4.14
UMP
substrate inhibition above 0.2 mM
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.4.14
0.12
-
CMP
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
0.5
-
CMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.5
-
UMP
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
1.5
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.022
-
CMP
pH 7.4, 37C
Homo sapiens
2.7.4.14
0.044
-
UMP
pH 7.4, 37C
Homo sapiens
2.7.4.14
0.045
-
UMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.07
-
CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.14
-
UMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.15
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.2
-
CMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
0.2
-
UMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
0.26
-
ara-CMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.3
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.34
-
ara-CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
0.9
-
dCMP
pH 7.4, 37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
1.1
-
dCMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
2
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
2
-
cCMP
pH 7.4, 37C
Dictyostelium sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + dCDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
ADP + UDP
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + ara-CMP
-
645162
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + ara-CMP
-
645162
Dictyostelium sp.
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
-
645162
Homo sapiens
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate
-
-
-
?
2.7.4.14
ATP + CMP
-
645162
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
-
645162
Dictyostelium sp.
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645162
Dictyostelium sp.
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
2.7.4.14
ATP + UMP
-
645162
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645162
Dictyostelium sp.
ADP + UDP
-
-
-
-
2.7.4.14
ATP + UMP
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
645162
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site
645162
Homo sapiens
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.14
10
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
14
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, GST fusion UMP/CMP kinase
Homo sapiens
2.7.4.14
22
-
dCMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
30
-
dCMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
36
-
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
45
-
ara-CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
72
-
CMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
73
-
dCMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
92
-
UMP
pH 7.4, 37C, GST fusion UMP-CMP kinase
Homo sapiens
2.7.4.14
130
-
CMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
130
-
UMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
150
-
ara-CMP
pH 7.4, 37C, His-tagged UMP-CMP kinase
Homo sapiens
2.7.4.14
217
-
UMP
pH 7.4, 37C
Dictyostelium sp.
2.7.4.14
410
-
CMP
pH 7.4, 37C
Dictyostelium sp.