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Literature summary extracted from

  • Pasti, C.; Gallois-Montbrun, S.; Munier-Lehmann, H.; Veron, M.; Gilles, A.M.; Deville-Bonne, D.
    Reaction of human UMP-CMP kinase with natural and analog substrates (2003), Eur. J. Biochem., 270, 1784-1790.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.14 expression of His-tagged UMP-CMP kinase and UMP-CMP kinase fusion protein with glutathione S-transferase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 CMP substrate inhibition above 0.2 mM Homo sapiens
2.7.4.14 UMP substrate inhibition above 0.2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.02
-
 UMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.022
-
 CMP pH 7.4, 37°C Homo sapiens
2.7.4.14 0.044
-
 UMP pH 7.4, 37°C Homo sapiens
2.7.4.14 0.045
-
 UMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.07
-
 CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 0.14
-
 UMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 0.15
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.2
-
 CMP pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 0.2
-
 UMP pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 0.26
-
 ara-CMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.3
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 0.34
-
 ara-CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 0.9
-
 dCMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 1.1
-
 dCMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 2
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 2
-
 cCMP pH 7.4, 37°C Dictyostelium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.14 ATP + CMP Homo sapiens the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP Homo sapiens the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies ADP + dCDP
-
 ?
2.7.4.14 ATP + UMP Homo sapiens the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Dictyostelium sp.
-
-
-
2.7.4.14 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + ara-CMP
-
 Homo sapiens ADP + ara-CDP
-
 ?
2.7.4.14 ATP + ara-CMP
-
 Dictyostelium sp. ADP + ara-CDP
-
 ?
2.7.4.14 ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
-
 Homo sapiens ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate
-
 ?
2.7.4.14 ATP + cCMP
-
 Dictyostelium sp. ?
-
 ?
2.7.4.14 ATP + CMP
-
 Homo sapiens ADP + CDP
-
 ?
2.7.4.14 ATP + CMP
-
 Dictyostelium sp. ADP + CDP
-
 ?
2.7.4.14 ATP + CMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + dCDP
-
 ?
2.7.4.14 ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
 Dictyostelium sp. ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
 ?
2.7.4.14 ATP + UMP
-
 Homo sapiens ADP + UDP
-
 ?
2.7.4.14 ATP + UMP
-
 Dictyostelium sp. ADP + UDP
-
 ?
2.7.4.14 ATP + UMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + UDP
-
 ?
2.7.4.14 additional information the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.14 10
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 14
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, GST fusion UMP/CMP kinase Homo sapiens
2.7.4.14 22
-
 dCMP pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 30
-
 dCMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 36
-
 beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, His-tagged UMP-CMP kinase Homo sapiens
2.7.4.14 45
-
 ara-CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 72
-
 CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 73
-
 dCMP pH 7.4, 37°C, His-tagged UMP-CMP kinase Homo sapiens
2.7.4.14 92
-
 UMP pH 7.4, 37°C, GST fusion UMP-CMP kinase Homo sapiens
2.7.4.14 130
-
 CMP pH 7.4, 37°C, His-tagged UMP-CMP kinase Homo sapiens
2.7.4.14 130
-
 UMP pH 7.4, 37°C, His-tagged UMP-CMP kinase Homo sapiens
2.7.4.14 150
-
 ara-CMP pH 7.4, 37°C, His-tagged UMP-CMP kinase Homo sapiens
2.7.4.14 217
-
 UMP pH 7.4, 37°C Dictyostelium sp.
2.7.4.14 410
-
 CMP pH 7.4, 37°C Dictyostelium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.14 0.12
-
 CMP pH 7.4, 37°C, GST fusion UMP/CMP kinase Homo sapiens
2.7.4.14 0.5
-
 CMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.5
-
 UMP pH 7.4, 37°C, GST fusion UMP/CMP kinase Homo sapiens
2.7.4.14 1.5
-
 UMP pH 7.4, 37°C, His-tagged UMP/CMP kinase Homo sapiens