BRENDA - Enzyme Database

Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates

Liou, J.Y.; Dutschman, G.E.; Lam, W.; Jiang, Z.; Cheng, Y.C.; Cancer Res. 62, 1624-1631 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
dCTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
TTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
UTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.015
-
CMP
37C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.023
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.067
-
UMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.069
-
UMP
37C, 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.204
-
ATP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.211
-
CMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.228
-
beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
37C
Homo sapiens
2.7.4.14
0.25
-
L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.272
-
beta-D-2',3'-dideoxy-CMP
37C
Homo sapiens
2.7.4.14
0.354
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.494
-
L-(-)-2',3'-dideoxy-3'-thia-CMP
37C
Homo sapiens
2.7.4.14
0.513
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.581
-
gemcitabine monophosphate
37C
Homo sapiens
2.7.4.14
0.697
-
beta-L-2',3'-dideoxy-CMP
37C
Homo sapiens
2.7.4.14
0.917
-
ara-CMP
37C
Homo sapiens
2.7.4.14
1.037
-
beta-D-2',3'-dideoxy-CMP
37C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + dCDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + UDP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
HeLa-S3 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + ara-CMP
-
645161
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + beta-D-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-dioxolane-cytidine
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + CMP
-
645161
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645161
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dCMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + gemcitabine monophosphate
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645161
Homo sapiens
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + UMP
-
645161
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
CTP + CMP
-
645161
Homo sapiens
CDP + CDP
-
-
-
?
2.7.4.14
dATP + CMP
-
645161
Homo sapiens
dADP + CDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645161
Homo sapiens
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + dCMP
-
645161
Homo sapiens
dCDP + dCDP
-
-
-
?
2.7.4.14
dCTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
2.7.4.14
dGTP + CMP
-
645161
Homo sapiens
dGDP + CDP
-
-
-
?
2.7.4.14
dGTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
2.7.4.14
GTP + CMP
-
645161
Homo sapiens
GDP + CDP
-
-
-
?
2.7.4.14
GTP + dCMP
-
645161
Homo sapiens
GDP + dCDP
-
-
-
?
2.7.4.14
GTP + UMP
-
645161
Homo sapiens
GDP + UDP
-
-
-
?
2.7.4.14
TTP + CMP
-
645161
Homo sapiens
TDP + CDP
-
-
-
?
2.7.4.14
TTP + dCMP
-
645161
Homo sapiens
TDP + dCMP
-
-
-
?
2.7.4.14
TTP + UMP
-
645161
Homo sapiens
TDP + UDP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
dCTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
TTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
2.7.4.14
UTP
inhibits reaction with ATP and UMP, CMP or dCMP
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.015
-
CMP
37C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.023
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.067
-
UMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.069
-
UMP
37C, 228-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.204
-
ATP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.211
-
CMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.228
-
beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
37C
Homo sapiens
2.7.4.14
0.25
-
L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
37C, His-tagged UMP/CMP kinase
Homo sapiens
2.7.4.14
0.272
-
beta-D-2',3'-dideoxy-CMP
37C
Homo sapiens
2.7.4.14
0.354
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.494
-
L-(-)-2',3'-dideoxy-3'-thia-CMP
37C
Homo sapiens
2.7.4.14
0.513
-
dCMP
37C, 196-aa enzyme form, in presence of 8 mM MgATP2-
Homo sapiens
2.7.4.14
0.581
-
gemcitabine monophosphate
37C
Homo sapiens
2.7.4.14
0.697
-
beta-L-2',3'-dideoxy-CMP
37C
Homo sapiens
2.7.4.14
0.917
-
ara-CMP
37C
Homo sapiens
2.7.4.14
1.037
-
beta-D-2',3'-dideoxy-CMP
37C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytoplasm
-
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + dCDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
ADP + UDP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
HeLa-S3 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + ara-CMP
-
645161
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + beta-D-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-2',3'-dideoxy-CMP + H2O
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + beta-L-dioxolane-cytidine
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + CMP
-
645161
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + CMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645161
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dCMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + gemcitabine monophosphate
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
645161
Homo sapiens
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
?
2.7.4.14
ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
-
645161
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + UMP
-
645161
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
ATP + UMP
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
645161
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
CTP + CMP
-
645161
Homo sapiens
CDP + CDP
-
-
-
?
2.7.4.14
dATP + CMP
-
645161
Homo sapiens
dADP + CDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645161
Homo sapiens
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + dCMP
-
645161
Homo sapiens
dCDP + dCDP
-
-
-
?
2.7.4.14
dCTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
2.7.4.14
dGTP + CMP
-
645161
Homo sapiens
dGDP + CDP
-
-
-
?
2.7.4.14
dGTP + UMP
-
645161
Homo sapiens
dGDP + UDP
-
-
-
?
2.7.4.14
GTP + CMP
-
645161
Homo sapiens
GDP + CDP
-
-
-
?
2.7.4.14
GTP + dCMP
-
645161
Homo sapiens
GDP + dCDP
-
-
-
?
2.7.4.14
GTP + UMP
-
645161
Homo sapiens
GDP + UDP
-
-
-
?
2.7.4.14
TTP + CMP
-
645161
Homo sapiens
TDP + CDP
-
-
-
?
2.7.4.14
TTP + dCMP
-
645161
Homo sapiens
TDP + dCMP
-
-
-
?
2.7.4.14
TTP + UMP
-
645161
Homo sapiens
TDP + UDP
-
-
-
?