BRENDA - Enzyme Database

Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli

Schultz, C.P.; Ylisastigui-Pons, L.; Serina, L.; Sakamoto, H.; Mantsch, H.H.; Neuhard, J.; Barzu, O.; Gilles, A.M.; Arch. Biochem. Biophys. 340, 144-153 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.035
-
CMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.04
-
CMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.094
-
dCMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.12
-
ATP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
2.7.4.25
0.33
-
dCMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.36
-
araCMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.37
-
ara-CMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
2.7.4.25
0.37
-
araCMP
pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.93
-
UMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
3.6
-
UMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.25
Bacillus subtilis
-
-
-
2.7.4.25
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.25
-
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + ara-CMP
-
645159
Escherichia coli
ADP + ara-CDP
-
-
-
-
2.7.4.25
ATP + ara-CMP
ara-CMP is phosphorylated at rate which represents 14% of that with CMP
645159
Bacillus subtilis
ADP + ara-CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645159
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645159
Escherichia coli
ADP + dCDP
-
-
-
-
2.7.4.25
ATP + dCMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + GTP
GTP is a poor substrate with Bacillus subtilis CMP kinase
645159
Bacillus subtilis
ADP + GDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645159
Escherichia coli
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is phosphorylated at rate which represents 10% of that with CMP
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
2.7.4.25
dATP + CMP
-
645159
Escherichia coli
dADP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
poor substrate
645159
Escherichia coli
GDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
ATP is equally effective as ATP
645159
Bacillus subtilis
GDP + CDP
-
-
-
-
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
48
-
midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP
Escherichia coli
2.7.4.25
48.5
57.8
the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5C, the melting temperature of CMP kinae in the presence of ATP is at 57.8C, the melting temperature of CMP kinae in the presence of CMP is at 50.5C
Bacillus subtilis
2.7.4.25
49
-
midpoint denaturation temperature in absence of nucleotide substrates
Bacillus subtilis
2.7.4.25
49
-
midpoint denaturation temperature in presence of CMP
Escherichia coli
2.7.4.25
51
-
midpoint denaturation temperature in presence of CMP
Bacillus subtilis
2.7.4.25
58
-
midpoint denaturation temperature in presence of ATP
Bacillus subtilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.035
-
CMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.04
-
CMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.094
-
dCMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.12
-
ATP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
2.7.4.25
0.33
-
dCMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.36
-
araCMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
0.37
-
ara-CMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH
Bacillus subtilis
2.7.4.25
0.37
-
araCMP
pH 7.4, 30C
Bacillus subtilis
2.7.4.25
0.93
-
UMP
pH 7.4, 30C
Escherichia coli
2.7.4.25
3.6
-
UMP
at 30C in 50mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1mM phosphoenolpyruvate, 0.2 mM NADH; pH 7.4, 30C
Bacillus subtilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + ara-CMP
-
645159
Escherichia coli
ADP + ara-CDP
-
-
-
-
2.7.4.25
ATP + ara-CMP
ara-CMP is phosphorylated at rate which represents 14% of that with CMP
645159
Bacillus subtilis
ADP + ara-CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645159
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645159
Escherichia coli
ADP + dCDP
-
-
-
-
2.7.4.25
ATP + dCMP
CMP and dCMP are the best phosphate acceptors
645159
Bacillus subtilis
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + GTP
GTP is a poor substrate with Bacillus subtilis CMP kinase
645159
Bacillus subtilis
ADP + GDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645159
Escherichia coli
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is phosphorylated at rate which represents 10% of that with CMP
645159
Bacillus subtilis
ADP + UDP
-
-
-
?
2.7.4.25
dATP + CMP
-
645159
Escherichia coli
dADP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
poor substrate
645159
Escherichia coli
GDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
ATP is equally effective as ATP
645159
Bacillus subtilis
GDP + CDP
-
-
-
-
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
48
-
midpoint denaturation temperature in absence of nucleotide substrates or in presence of ATP
Escherichia coli
2.7.4.25
48.5
57.8
the melting temperature of CMP kinae in the absence of nucleotide substrates is at 48.5C, the melting temperature of CMP kinae in the presence of ATP is at 57.8C, the melting temperature of CMP kinae in the presence of CMP is at 50.5C
Bacillus subtilis
2.7.4.25
49
-
midpoint denaturation temperature in absence of nucleotide substrates
Bacillus subtilis
2.7.4.25
49
-
midpoint denaturation temperature in presence of CMP
Escherichia coli
2.7.4.25
51
-
midpoint denaturation temperature in presence of CMP
Bacillus subtilis
2.7.4.25
58
-
midpoint denaturation temperature in presence of ATP
Bacillus subtilis