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Literature summary extracted from

  • Okajima, T.; Fukamizo, T.; Goto, S.; Fukui, T.; Tanizawa, K.
    Exchange of nucleoside monophosphate-binding domains in adenylate kinase and UMP/CMP kinase (1998), J. Biochem., 124, 359-367.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.14 additional information two types of chimeric enzymes have been constructed by genetic engineering of chicken cytosolic adenylate kinase and porcine brain UMP/CMP kinase. One designated as UAU carries an AMP-binding domain of AK in the remaining body of UMP/CMP kinase, and the other, designated as AUA, carries a UMP/CMP-binding domain in the remaining body of adenylate kinase. UAU is 4fold more active with AMP, 40fold less active with UMP, and 4fold less active with CMP than the parental UMP/CMP kinase, although AUA has considerably lowered reactivity for both AMP and UMP. AUA has a Tm-value 11°C lower than adenylate kinase, whereas UAU has a Tm-value similar to that of UMP/CMP kinase. Expression in Escherichia coli JM109 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 brain
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + CMP
-
 Sus scrofa ADP + CDP
-
 ?
2.7.4.14 ATP + UMP
-
 Sus scrofa ADP + UDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.14 50
-
 midpoint denaturation temperature of the AUA chimeric enzyme Sus scrofa
2.7.4.14 51
-
 midpoint denaturation temperature of UMP/CMP kinase Sus scrofa
2.7.4.14 52
-
 midpoint denaturation temperature of the UAU chimeric enzyme Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.14 0.65
-
 UMP pH 7.0, 25°C, AUA chimeric enzyme Sus scrofa
2.7.4.14 13.7
-
 UMP pH 7.0, 25°C, UAU chimeric enzyme Sus scrofa
2.7.4.14 174
-
 UMP pH 7.0, 25°C, UMP-CMP kinase Sus scrofa